Velasco-Lezama R, Tapia-Aguilar R, Luna-Acevedo P, Cruz-Islas E, Garza-Garza D
Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, México, D.F., Mexico.
Rev Latinoam Microbiol. 1995 Apr-Jun;37(2):135-46.
The pathogenicity of the L, P and E strains of C. neoformans was studied in 3-5 weeks old CD1 male mice. Cell suspensions containing the most (L), and the least (E) pathogenic strains were inoculated intracerebrally (IC), and intraperitoneally (IP). After 14 days total and differential counts were made for erythrocytes, leukocytes and platelets, yeasts from infected organs were recovered in Sabouraud Dextrose Agar (SDA). The capsular material from C. neoformans was used to stimulate in vivo and in vitro platelet production. Mice inoculated with the L strain, showed increase of neutrophils and platelets, decrease of lymphocytes, the yeast was recovered from spleen, liver and lungs and from brain of mice injected by the IC route. In contrast, strain E produced an increase of neutrophils, reduction of lymphocytes, and was only isolated from the brain of mice inoculated IC. The capsular material stimulated the production and maturation of megakaryocytes only in vitro. The development of the infection and the yeast dissemination were associated with increases in the number of platelets, probably as a result of the stimulant effect of the capsular material on the maturation of megakaryocytes.
在3至5周龄的CD1雄性小鼠中研究了新型隐球菌L、P和E菌株的致病性。将含有致病性最强（L）和最弱（E）菌株的细胞悬液进行脑内（IC）和腹腔内（IP）接种。14天后，对红细胞、白细胞和血小板进行总数和分类计数，并在沙氏葡萄糖琼脂（SDA）中从受感染器官中分离出酵母。使用新型隐球菌的荚膜物质刺激体内和体外血小板生成。接种L菌株的小鼠显示中性粒细胞和血小板增加，淋巴细胞减少，通过IC途径注射的小鼠的脾脏、肝脏、肺和脑中可分离出酵母。相比之下，E菌株导致中性粒细胞增加，淋巴细胞减少，并且仅从IC接种小鼠的脑中分离得到。荚膜物质仅在体外刺激巨核细胞的产生和成熟。感染的发展和酵母播散与血小板数量增加有关，这可能是荚膜物质对巨核细胞成熟的刺激作用的结果。
