Staib F, Mishra S K
Zentralbl Bakteriol Orig A. 1975 Jan;230(1):81-5.
The results briefly presented here highlight some of the observations met with during the course of a study aimed at finding out differential pathogenic behavior of the two strains of Cryptococcus neoformans i.e. W71/A117 and W2/A94 with special reference to cryptococcoma formation. Both strains were isolated from bird excreta but differed in their gross and microscopic morphology. Groups of male albino mice NMRI were separately inoculated, intramuscularly, with a comparable dose of the two strains. All the 50 animals challenged with W71/A117 developed macroscopically distinct cryptococcoma of variable size, and fatally progressive disease, involving most of the internal organs, namely, brain, heart, lung, liver, spleen and kidneys. On the contrary, only 34 of the 50 mice infected with the strain W2/A94 showed cryptococcoma formation at the site of inoculation, which were comparatively much smaller in size and gradually diminished. None of the animals, observed over a period of 93 days, died, and showed any sign of metastasis. In another series of experiment, only one instance of mortality was observed in a group of 80 mice inoculated intraperitoneally with about 2 X 10(7) viable cells of this strain. However, the fungus could be recovered, in a majority of cases, only from the brain of animals sacrificed after one month, though most of them showed no sign of sickness. The number of mice yielding positive cultures gradually decreased, and after 87 days the fungus could not be isolated from any organ. The high morbidity and mortality in mice caused by strains W71/A117 was significantly lowered when the animals were infected intramuscularly 3 months ago with the strain W2/A94. After an observation period of 61 days, 91% of the double infected animals were still alive in comparison to 23% survival among the animals challenged with the strain W71/A117 only.
此处简要呈现的结果突出了在一项研究过程中所观察到的一些情况。该研究旨在探究新型隐球菌的两株菌株即W71/A117和W2/A94的不同致病行为，尤其涉及隐球菌瘤的形成。这两株菌株均从鸟类排泄物中分离得到，但在大体和微观形态上有所不同。将雄性NMRI白化小鼠分组，分别肌肉注射同等剂量的这两株菌株。用W71/A117攻击的所有50只动物都出现了肉眼可见的大小各异的明显隐球菌瘤，并患上致命的进行性疾病，累及大多数内部器官，即脑、心脏、肺、肝脏、脾脏和肾脏。相反，感染W2/A94菌株的50只小鼠中只有34只在接种部位出现隐球菌瘤形成，其尺寸相对小得多且逐渐缩小。在93天的观察期内，没有动物死亡，也没有出现转移迹象。在另一系列实验中，在一组80只经腹腔注射约2×10⁷个该菌株活细胞的小鼠中，仅观察到一例死亡。然而，在大多数情况下，仅在一个月后处死的动物的脑中才能分离出真菌，尽管它们大多数没有患病迹象。培养呈阳性的小鼠数量逐渐减少，87天后在任何器官中都无法分离出该真菌。当动物在3个月前经肌肉注射感染W2/A94菌株后，由W71/A117菌株引起的小鼠高发病率和死亡率显著降低。在61天的观察期后，双重感染的动物中有91%仍然存活，而仅用W71/A117菌株攻击的动物的存活率为23%。
