两种转化试验（C3H/10T1/2和SHE细胞）中环氧丙烷和环氧氯丙烷效应的比较。

Comparison of propylene oxide and epichlorohydrin effects in two transformation tests (C3H/10T1/2 and SHE cells).

作者信息

Kolman A, Dusinská M

机构信息

Department of Radiobiology, Stockholm University, Sweden.

出版信息

Toxicol Lett. 1995 Nov 15;81(2-3):213-21. doi: 10.1016/0378-4274(95)03442-0.

DOI:10.1016/0378-4274(95)03442-0

PMID:8553377

Abstract

The neoplastic cell transformation induced by propylene oxide (PO) and epichlorohydrin (ECH) was studied in two in vitro assays, mouse embryo fibroblasts (C3H/10T1/2) and Syrian hamster embryo (SHE) cells. In C3H/10T1/2 cells treated with PO (2.5-10 mM), the transformation frequencies were enhanced about 2-4 times in the presence of 12-O-tetradecanoylphorbol-13-acetate (TPA), compared with the transformation frequencies in the absence of TPA. In SHE cells, an even higher increase (about 6-9 times) was reached at concentrations of 2.5-20 mM. The presence of TPA strongly influenced the ability of ECH to induce the morphological transformation at low-moderate concentrations (0.25-1 mM). At the highest concentrations applied, 1 mM in C3H/10T1/2 cells and 0.5 mM in SHE cells, 41- and 4-fold increases, respectively, were observed. In C3H/10T1/2 cells, the rad-equivalence (rad/mMh) of PO and ECH in the presence of TPA was calculated to be 36 +/- 8 and 296 +/- 65 (mean +/- S.E.), respectively.

摘要

在两种体外试验中，即小鼠胚胎成纤维细胞（C3H/10T1/2）和叙利亚仓鼠胚胎（SHE）细胞试验中，研究了环氧丙烷（PO）和环氧氯丙烷（ECH）诱导的肿瘤细胞转化。在用PO（2.5 - 10 mM）处理的C3H/10T1/2细胞中，与不存在12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）时的转化频率相比，在TPA存在的情况下，转化频率提高了约2 - 4倍。在SHE细胞中，当浓度为2.5 - 20 mM时，转化频率甚至有更高的增加（约6 - 9倍）。TPA的存在强烈影响了ECH在低 - 中等浓度（0.25 - 1 mM）下诱导形态转化的能力。在最高应用浓度下，C3H/10T1/2细胞中为1 mM，SHE细胞中为0.5 mM，分别观察到增加了41倍和4倍。在C3H/10T1/2细胞中，TPA存在时PO和ECH的拉德当量（rad/mMh）分别计算为36 ± 8和296 ± 65（平均值 ± 标准误）。