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[气相色谱法测定脂质成分中各高分子量脂肪酸]

[Gas chromatographic determination of individual high molecular weight fatty acids in the composition of lipids].

作者信息

Rivis I F, Danilik B B

出版信息

Ukr Biokhim Zh (1978). 1995 Jul-Aug;67(4):96-9.

PMID:8553481
Abstract

A rapid and precise method is suggested for quantitative determination of highly molecular fatty acids in a sample either in the esterified (mono-, di- and triglycerides, cholesterol ethers, and phospholipids) or in the non-esterified state, as well as like salts of fatty acids (soaps). Methyl ethers of fatty acids have been obtained by immediate methanolysis of the sample studied. The method advantages are both indices of the amount of the internal standard added to the sample of concern and possibility to determine highly molecular fatty acids in any sample taken either from plants or from animals.

摘要

本文提出了一种快速精确的方法,用于定量测定样品中酯化状态(单、二和甘油三酯、胆固醇醚和磷脂)或非酯化状态的高分子脂肪酸,以及脂肪酸盐(肥皂)。通过对所研究样品进行直接甲醇解得到脂肪酸甲酯。该方法的优点在于所添加到相关样品中的内标物量的指标,以及能够测定取自植物或动物的任何样品中的高分子脂肪酸。

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