Kempkes B, Pawlita M, Zimber-Strobl U, Eissner G, Laux G, Bornkamm G W
GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Klinische Molekularbiologie und Tumorgenetik, München, Germany.
Virology. 1995 Dec 20;214(2):675-9. doi: 10.1006/viro.1995.0084.
Epstein-Barr virus nuclear antigen 2 (EBNA2) is a transcriptional activator of viral and cellular genes involved in B cell transformation by EBV and is targeted to EBV responsive promoters through interaction with cellular DNA binding proteins such as RBP-J kappa. To develop a conditional system in which the function of EBNA2 can be switched on and off, we have fused the hormone binding domain of the estrogen receptor to the N- or C-terminus of EBNA2. Here we show that after transient or stable transfer of these chimerical EBNA2 genes into human B cell lymphoma lines, transactivation of LMP1, TP1, and TP2 promoter constructs, expression of the cell surface markers CD21 and CD23, and binding of EBNA2 to its cellular partner RBP-J kappa are dependent on the presence of estrogen. The EBNA2 fusion proteins proved to be virtually inactive in the absence of hormone.
爱泼斯坦-巴尔病毒核抗原2（EBNA2）是一种病毒和细胞基因的转录激活因子，参与EBV介导的B细胞转化，它通过与细胞DNA结合蛋白如RBP-Jκ相互作用靶向EBV反应性启动子。为了构建一个能使EBNA2功能开启和关闭的条件系统，我们将雌激素受体的激素结合结构域融合到EBNA2的N端或C端。在此我们表明，将这些嵌合EBNA2基因瞬时或稳定转入人B细胞淋巴瘤系后，LMP1、TP1和TP2启动子构建体的反式激活、细胞表面标志物CD21和CD23的表达以及EBNA2与其细胞伴侣RBP-Jκ的结合均依赖于雌激素的存在。事实证明，在没有激素的情况下，EBNA2融合蛋白几乎没有活性。
