Bulatov A A, Kiseleva A G, Gorshkova T V, Akopova N B, Marova E I
Vopr Med Khim. 1995 Sep-Oct;41(5):19-23.
Specific radioimmunoassay was developed for detection free glycoprotein hormone alpha-subunit (AS), by using AS prepared from highly purified human thyroid-stimulating hormone (TSH). The assay showed that the basal content of free serum AS in the majority of 24 females with hormonally pituitary tumors secreting somatotropic hormone (STH, growth hormone), prolactin or either and in 24 females with unfunctioning pituitary tumors was significantly higher the mean one in 12 females matched for the same age who had no endocrine diseases (control). The cultured cells of STH- and P-secreting tumors released excessive quantities of free AS along with STH and P into the medium with low glycoprotein holo-hormone levels. The content of AS changed little in 12 patients with non-functioning pituitary tumors despite the greatly increased serum TSH levels in response to the hypothalamic stimulator thyroid-releasing hormone. The findings suggest that free AS secreted into blood in excess despite hypothalamic control may be regarded as a biochemical marker of pituitary tumors.
利用从高度纯化的人促甲状腺激素（TSH）制备的α亚基（AS），开发了用于检测游离糖蛋白激素α亚基的特异性放射免疫测定法。该测定法显示，在24名患有分泌生长激素（STH）、催乳素或两者皆有的激素性垂体肿瘤的女性以及24名患有无功能垂体肿瘤的女性中，大多数人血清游离AS的基础含量显著高于12名年龄匹配且无内分泌疾病的女性（对照组）的平均水平。分泌STH和催乳素的肿瘤培养细胞将过量的游离AS与STH和催乳素一起释放到糖蛋白全激素水平较低的培养基中。12名无功能垂体肿瘤患者的AS含量变化不大，尽管对下丘脑刺激物促甲状腺激素释放激素反应后血清TSH水平大幅升高。这些发现表明，尽管有下丘脑控制，但过量分泌到血液中的游离AS可被视为垂体肿瘤的生化标志物。
