Prokof'ev V N, Mogil'nitskaia L V, Lukash A I
Vopr Med Khim. 1995 Sep-Oct;41(5):39-42.
Chemiluminescence analysis was used to examine the yeast cell-activation of rat whole blood neutrophilic leukocytes in rats after their pre-exposure to 3-hour hypoxia (9,000 m above sea level) and two-hour hyperoxia (0.3 MPa), as well as their resultant effects. The hypoxia was found to cause a significant (42%) reduction in the length of the latent period, suggesting that it activates the phagocytic activity of neutrophilic leukocytes. At the same time hyperoxia leads to a delayed response of competent cells to yeast cell stimulation. On subsequent exposure to hypoxia and hyperoxia, drastic inhibition of drastic cellular activation occurred, which manifested itself by a 62% increase in the length of the latent period and by a 38% rise in the appearance time of luminescence peak. Thus, the findings suggest that it is essential to bear in mind the fact that phagocytosis can decrease if oxygen is overdosed during hyperbaric oxygenation and in hypoxic states.
采用化学发光分析法检测大鼠全血中性粒细胞在预先暴露于3小时低氧（海拔9000米）和2小时高氧（0.3MPa）后对酵母细胞的激活情况及其产生的影响。结果发现，低氧导致潜伏期时长显著缩短（42%），这表明低氧激活了中性粒细胞的吞噬活性。与此同时，高氧导致感受态细胞对酵母细胞刺激的反应延迟。在随后再次暴露于低氧和高氧时，细胞的剧烈激活受到强烈抑制，表现为潜伏期时长增加62%，发光峰值出现时间延迟38%。因此，这些研究结果表明，必须牢记这样一个事实，即在高压氧疗和低氧状态下，如果氧气过量，吞噬作用可能会降低。
