Eschenbach C
Klin Wochenschr. 1982 Apr 1;60(7):357-64. doi: 10.1007/BF01721626.
The oxidative metabolism, and thus the bactericidal activity, of neutrophil granulocytes can be evaluated by measurement of the luminol chemiluminescence during phagocytosis. The method described requires only a small number of separated cells (0.3 X 10(6)); continuous measurement in the physiologic temperature range is possible. The procedure is described and results of polymorphonuclear leukocytes from healthy adults and umbilical cord blood from healthy newborns are given. Quantitative variations in the single components (number of granulocytes, number of phagocytic particles, concentration of luminol) and the measuring volume are investigated. The significance of the chosen parameters "photon emission during defined time of measurement" and "time of maximum photon emission per unit time" used for evaluation is shown by comparison of the results from the influence of phorbol myristate acetate on the luminol chemiluminescence of phagocytosing granulocytes. The method described enables the evaluation of the capacity and the temporal course of stimulation of the oxidative metabolism of polymorphonuclear granulocytes in phagocytosis.
中性粒细胞的氧化代谢及杀菌活性可通过吞噬过程中鲁米诺化学发光的测量来评估。所述方法仅需少量分离细胞（0.3×10⁶）；可在生理温度范围内进行连续测量。文中描述了该程序，并给出了健康成年人多形核白细胞和健康新生儿脐带血的结果。研究了单个组分（粒细胞数量、吞噬颗粒数量、鲁米诺浓度）和测量体积的定量变化。通过比较佛波酯肉豆蔻酸酯对吞噬粒细胞鲁米诺化学发光影响的结果，显示了用于评估的所选参数“测量特定时间内的光子发射”和“单位时间内最大光子发射时间”的意义。所述方法能够评估多形核粒细胞在吞噬过程中氧化代谢的刺激能力及其时间进程。
