GUF1，一个在芽殖酵母中编码一种新型进化保守GTP酶的基因。

GUF1, a gene encoding a novel evolutionarily conserved GTPase in budding yeast.

作者信息

Kiser G L, Weinert T A

机构信息

Molecular and Cellular Biology Department, University of Arizona, Tucson 85721, USA.

出版信息

Yeast. 1995 Oct;11(13):1311-6. doi: 10.1002/yea.320111312.

DOI:10.1002/yea.320111312

PMID:8553703

Abstract

While sequencing a region of chromosome IV adjacent to the checkpoint gene MEC3, we identified a gene we call GUF1 (GTPase of Unknown Function), which predicts a 586 amino acid GTPase of the elongation factor-type class. The predicted Guf1p protein bears striking sequence similarity to both LepA from Escherichia coli (43% identical) and LK1236.1 from Caenorhabditis elegans (42% identical). Analysis of both a guf1 delta deletion and a putative constitutive-activating mutant (GUF1HG) revealed that GUF1 is not essential nor did mutant cells reveal any marked phenotype.

摘要

在对与检查点基因MEC3相邻的第四条染色体区域进行测序时，我们鉴定出一个基因，我们将其命名为GUF1（功能未知的GTP酶），它预测是一个含有586个氨基酸的延伸因子型GTP酶。预测的Guf1p蛋白与大肠杆菌的LepA（43%相同）和秀丽隐杆线虫的LK1236.1（42%相同）都有显著的序列相似性。对guf1缺失突变体和一个假定的组成型激活突变体（GUF1HG）的分析表明，GUF1不是必需的，突变细胞也没有表现出任何明显的表型。