Jonassen T, Marbois B N, Kim L, Chin A, Xia Y R, Lusis A J, Clarke C F
Department of Chemistry and Biochemistry, University of California, Los Angeles 90095, USA.
Arch Biochem Biophys. 1996 Jun 15;330(2):285-9. doi: 10.1006/abbi.1996.0255.
We recently identified the Saccharomyces cerevisiae COQ7 gene and showed that its product affects one or more monoxygenase steps in the synthesis of ubiquinone. Other investigators have independently isolated the yeast COQ7 gene as CAT5 and identified it as a gene necessary for the derepression of gluconeogenic enzymes in yeast. In the present study, a homolog of the yeast COQ7 (CAT5) gene was isolated from a rat testis cDNA library by functional complementation of a coq7 deletion mutant of S. cerevisiae. The resulting cDNA clones contained a 0.8-kb insert with an open reading frame encoding a 183-amino-acid polypeptide. The rat Coq7 amino acid sequence is 49% identical to that of yeast Coq7p and 58% identical to a C. elegans homolog over a 152-aa region. Sequence homology searches fail to identify any other significant homologies. The Coq7 gene was mapped to mouse chromosome 7, 7.6 +/- 3.6 cM proximal to the marker D7Mit7, by linkage analysis of an interspecific backcross. This region of chromosome 7 containing Coq7 is part of a linkage group conserved between mouse chromosome 7 and human chromosome 11p15.
我们最近鉴定出了酿酒酵母COQ7基因，并表明其产物影响泛醌合成中的一个或多个单加氧酶步骤。其他研究人员已独立地将酵母COQ7基因分离为CAT5，并将其鉴定为酵母中糖异生酶去阻遏所必需的基因。在本研究中，通过酿酒酵母coq7缺失突变体的功能互补，从大鼠睾丸cDNA文库中分离出酵母COQ7（CAT5）基因的一个同源物。所得的cDNA克隆包含一个0.8 kb的插入片段，带有一个编码183个氨基酸多肽的开放阅读框。大鼠Coq7氨基酸序列与酵母Coq7p的序列有49%的同一性，在152个氨基酸区域内与秀丽隐杆线虫的一个同源物有58%的同一性。序列同源性搜索未能鉴定出任何其他显著的同源性。通过种间回交的连锁分析，将Coq7基因定位到小鼠第7号染色体上，位于标记D7Mit7近端7.6 +/- 3.6 cM处。包含Coq7的第7号染色体的这个区域是小鼠第7号染色体和人类第11号染色体p15之间保守的连锁群的一部分。
