Maurice T C, Mazzucco C E, Ramanathan C S, Ryan B M, Warr G A, Puziss J W
Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, CT 06492, USA.
Yeast. 1998 Sep 15;14(12):1105-13. doi: 10.1002/(SICI)1097-0061(19980915)14:12<1105::AID-YEA313>3.0.CO;2-Y.
A paralog (intraspecies homolog) of the Saccharomyces cerevisiae YEF3 gene, encoding elongation factor-3, has been sequenced in the course of the yeast genome project, and identified by database searching; this gene has been designated HEF3. Bioinformatic and Northern blot analysis indicate that the HEF3 gene is not expressed during vegetative growth. Deletion of the HEF3 gene reveals no growth defects, nor any defects in mating or sporulation. A high copy 2 mu clone of HEF3 was constructed, and was shown to be unable to complement a null allele of yef3. Finally, an in vitro assay for ribosome-stimulated ATPase activity was performed with isogenic HEF3 and delta hef3 strains; no difference in biochemical activity could be detected in these strains. From these results, we conclude that the HEF3 gene does not encode a functional homolog of YEF3.
在酵母基因组计划过程中，对酿酒酵母YEF3基因（编码延伸因子-3）的一个旁系同源基因（种内同源基因）进行了测序，并通过数据库搜索得以鉴定；该基因被命名为HEF3。生物信息学和Northern印迹分析表明，HEF3基因在营养生长期间不表达。HEF3基因的缺失未显示出生长缺陷，也未显示出交配或孢子形成方面的任何缺陷。构建了HEF3的高拷贝2μ克隆，并证明其无法互补yef3的无效等位基因。最后，用同基因的HEF3和Δhef3菌株进行了核糖体刺激的ATP酶活性的体外测定；在这些菌株中未检测到生化活性的差异。根据这些结果，我们得出结论，HEF3基因不编码YEF3的功能同源物。
