Hertz L, Yager J Y, Juurlink B H
Saskatchewan Stroke Research Centre, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Int J Dev Neurosci. 1995 Oct;13(6):523-7. doi: 10.1016/0736-5748(95)00049-m.
Astrocyte cultures prepared from newborn mouse neopallium were grown for either one or three weeks (representing, respectively, immature and mature astrocytes) and then exposed to deprivation of substrate (glucose and amino acids) for up to 48 hr. Cultures which had been deprived of metabolic substrates for either 24, 30, 36 or 48 hr were examined for lactate dehydrogenase efflux into the medium (an indicator of cell death) and ATP content. Significant cell death in mature astrocytes began after 30 hr of incubation in the substrate-deprived medium, a time when ATP had fallen to approximately 10% of its initial value. Immature astrocytes survived on a substrate-free medium for 48 hr before there was any indication at all of cell death, and this corresponded to a time when ATP values had fallen to 5% of the initial values. These findings are compared to previous observations during simulated ischemia (substrate deprivation plus anoxia) when (1) there was a faster cell death and (2) cell death occurred at higher ATP levels.
从新生小鼠大脑新皮质制备的星形胶质细胞培养物分别培养1周或3周（分别代表未成熟和成熟的星形胶质细胞），然后使其暴露于缺乏底物（葡萄糖和氨基酸）的环境中长达48小时。对剥夺代谢底物24、30、36或48小时的培养物进行检测，以观察乳酸脱氢酶向培养基中的释放情况（细胞死亡的指标）以及ATP含量。在缺乏底物的培养基中孵育30小时后，成熟星形胶质细胞开始出现显著的细胞死亡，此时ATP已降至其初始值的约10%。未成熟星形胶质细胞在无底物培养基中存活48小时后才出现任何细胞死亡迹象，这与ATP值降至初始值的5%的时间相对应。这些发现与之前在模拟缺血（底物剥夺加缺氧）期间的观察结果进行了比较，当时（1）细胞死亡更快，（2）细胞死亡发生在较高的ATP水平。
