共聚焦显微镜检查和变色染料染色诊断微孢子虫性角膜炎
Diagnosis of microsporidial keratitis by confocal microscopy and the chromatrope stain.
作者信息
Shah G K, Pfister D, Probst L E, Ferrieri P, Holland E
机构信息
Department of Ophthalmology, University of Minnesota Hospital 55455-0501, USA.
出版信息
Am J Ophthalmol. 1996 Jan;121(1):89-91. doi: 10.1016/s0002-9394(14)70538-0.
PURPOSE
To illustrate the value of confocal microscopy and chromatrope stain in the diagnosis of microsporidial keratitis.
METHODS
In vivo confocal microscopy was performed on a man with the human immunodeficiency virus who had severe bilateral epithelial keratitis refractory to topical antibacterial medications. The results were compared to conjunctival scrapings stained with the chromatrope-based Weber stain.
RESULTS
Confocal microscopy demonstrated many small, intraepithelial opacities of the corneal epithelium, which were suggestive of Microsporidia. Results of the chromatrope stain of conjunctival scrapings confirmed the diagnosis of microsporidial keratitis.
CONCLUSIONS
Rapid diagnosis allowed prompt initiation of topical fumagillin, which permitted rapid, long-term control of the symptoms of microsporidial keratitis.
目的
阐述共聚焦显微镜检查和嗜色菌素染色在微孢子虫性角膜炎诊断中的价值。
方法
对一名感染人类免疫缺陷病毒且双侧严重上皮性角膜炎，局部使用抗菌药物治疗无效的男性患者进行活体共聚焦显微镜检查。将结果与用基于嗜色菌素的韦伯染色法染色的结膜刮片结果进行比较。
结果
共聚焦显微镜检查显示角膜上皮有许多小的上皮内混浊，提示为微孢子虫。结膜刮片的嗜色菌素染色结果证实了微孢子虫性角膜炎的诊断。
结论
快速诊断使得能够迅速开始局部使用烟曲霉素，从而实现对微孢子虫性角膜炎症状的快速、长期控制。
Zotero 插件