Jeong H G, Yun C H
Department of Environmental Science, Chosun University, Kwangju, Korea.
Biochem Biophys Res Commun. 1995 Dec 26;217(3):966-71. doi: 10.1006/bbrc.1995.2864.
The effect of myristicin on the expression of liver cytochrome P450s and its mRNA levels was examined in rats. Treatment of rats with myristicin (i.p., 500 mumol/kg) caused 2-20 fold increases in liver P450 1A1/2, 2B1/2, and 2E1 enzyme activities relative to controls. Immunoblot analysis using anti-rat liver P450 1A, 2B, and 2E1 showed that the increases in each of P450 protein levels by myristicin were consistent with those in enzyme activity levels. When increased levels of P450 mRNA by myristicin were examined by Northern blot analysis, levels of mRNA of P450 1A1/2 and 2B1/2 also increased. However, the level of P4502E1 mRNA did not increase. The total amount of spectrally detectable P450, also increased about 1.5-fold following treatment of myristicin. These results demonstrate that myristicin is an inducer of rat liver P450 1A1/2, 2B1/2, and 2E1, and that the induction involves increases in mRNA levels except in the case of P4502E1.
研究了肉豆蔻醚对大鼠肝脏细胞色素P450s表达及其mRNA水平的影响。给大鼠腹腔注射肉豆蔻醚（500 μmol/kg）后，相对于对照组，肝脏P450 1A1/2、2B1/2和2E1酶活性增加了2至20倍。使用抗大鼠肝脏P450 1A、2B和2E1进行免疫印迹分析表明，肉豆蔻醚使每种P450蛋白水平的增加与酶活性水平的增加一致。通过Northern印迹分析检测肉豆蔻醚使P450 mRNA水平升高的情况时，发现P450 1A1/2和2B1/2的mRNA水平也升高了。然而，P4502E1 mRNA水平并未升高。经肉豆蔻醚处理后，光谱可检测到的P450总量也增加了约1.5倍。这些结果表明，肉豆蔻醚是大鼠肝脏P450 1A1/2、2B1/2和2E1的诱导剂，且这种诱导作用除P4502E1外均涉及mRNA水平的升高。
