用于从接种食品中分离空肠弯曲菌的Oxyrase富集方法的评估。

Evaluation of Oxyrase enrichment method for isolation of Campylobacter jejuni from inoculated foods.

作者信息

Tran T T

机构信息

Division of Microbiological Studies, Food and Drug Administration, Washington, DC 20204, USA.

出版信息

Lett Appl Microbiol. 1995 Dec;21(6):345-7. doi: 10.1111/j.1472-765x.1995.tb01077.x.

DOI:10.1111/j.1472-765x.1995.tb01077.x

PMID:8554759

Abstract

Recovery limits were evaluated for Campylobacter jejuni in an existing Food and Drug Administration (FDA) enrichment broth (EB) formula supplemented with Oxyrase enzyme. Cultures of Camp. jejuni were inoculated into EB or EB containing 10% raw milk, raw oysters, crabmeat or mushrooms. After 24 and 48 h of enrichment, Camp. jejuni was isolated on four selective agars. No significant differences in recovery rates for Camp. jejuni were observed in the Oxyrase enrichment under normal atmosphere or in the existing FDA method under modified atmosphere. Increase of enrichment time from 24 to 48 h did not improve the recovery rates. However, the Oxyrase enrichment was cost effective, less time consuming, and simpler to perform than the established method.

摘要

在现有的美国食品药品监督管理局（FDA）增菌肉汤（EB）配方中添加氧化酶，对空肠弯曲菌的回收率进行了评估。将空肠弯曲菌培养物接种到EB或含有10%生牛奶、生牡蛎、蟹肉或蘑菇的EB中。增菌24小时和48小时后，在四种选择性琼脂上分离空肠弯曲菌。在正常大气条件下的氧化酶增菌或在改良大气条件下的现有FDA方法中，空肠弯曲菌的回收率没有显著差异。增菌时间从24小时增加到48小时并没有提高回收率。然而，与既定方法相比，氧化酶增菌具有成本效益、耗时更少且操作更简单。