用于乳酸菌和肠球菌菌株分型的随机扩增多态性DNA（RAPD）方案的开发。
Development of RAPD protocol for typing of strains of lactic acid bacteria and enterococci.
作者信息
Cocconcelli P S, Porro D, Galandini S, Senini L
机构信息
Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Piacenza, Italy.
出版信息
Lett Appl Microbiol. 1995 Dec;21(6):376-9. doi: 10.1111/j.1472-765x.1995.tb01085.x.
A protocol for typing strains of lactic acid bacteria and enterococci based on randomly amplified polymorphic DNA (RAPD) fragments has been developed. Using a single 10-mer primer, fingerprints were achieved without the need to isolate genomic DNA. Different conditions of DNA release and amplification were investigated in order to obtain reproducible results and high discrimination among strains. This RAPD protocol was successfully applied for the typing of strains belonging to the species Lactobacillus acidophilus, Lact. helveticus, Lact. casei, Lact. reuteri, Lact. plantarum, Enterococcus faecalis, Ent. faecium and Streptococcus thermophilus.
已开发出一种基于随机扩增多态性DNA（RAPD）片段对乳酸菌和肠球菌菌株进行分型的方案。使用单一的10聚体引物，无需分离基因组DNA即可获得指纹图谱。为了获得可重复的结果以及菌株间的高鉴别力，研究了不同的DNA释放和扩增条件。该RAPD方案已成功应用于嗜酸乳杆菌、瑞士乳杆菌、干酪乳杆菌、罗伊氏乳杆菌、植物乳杆菌、粪肠球菌、屎肠球菌和嗜热链球菌等菌株的分型。
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