根瘤菌科中大亚基核糖体DNA 5'端的变异性及一类新的短rRNA的存在

Variability of the 5'-end of the large subunit rDNA and presence of a new short class of rRNA in Rhizobiaceae.

作者信息

Evguenieva-Hackenberg E, Selenska-Pobell S

机构信息

Department of Genetics, University of Bayreuth, Germany.

出版信息

Lett Appl Microbiol. 1995 Dec;21(6):402-5. doi: 10.1111/j.1472-765x.1995.tb01092.x.

DOI:10.1111/j.1472-765x.1995.tb01092.x

PMID:8554768

Abstract

A highly variable region of DNA was found between positions 115 and 388 (Escherichia coli numeration) of the large subunit (ls) rRNA genes of 55 rhizobial and agrobacterial strains. In each case this heterogeneity was accompanied by the presence of a new rRNA species approximately 130 bp long. This novel rRNA species corresponded to the 5'-end of the ls rRNA genes. An additional rRNA processing site was located in the central region of the remaining ls rRNA of many of the Rhizobium leguminosarum and Rh. etli strains, and in all of the agrobacteria studied, excepting the type strain of Agrobacterium vitis NCPPB 3554 and Agrobacterium sp. strain ChAg4.

摘要

在55株根瘤菌和土壤杆菌菌株的大亚基（ls）rRNA基因的115至388位（大肠杆菌编号）之间发现了一个高度可变的DNA区域。在每种情况下，这种异质性都伴随着一种新的约130 bp长的rRNA物种的存在。这种新的rRNA物种对应于ls rRNA基因的5'端。在许多豌豆根瘤菌和埃氏根瘤菌菌株的其余ls rRNA的中央区域，以及在所有研究的土壤杆菌中，除了葡萄土壤杆菌NCPPB 3554型菌株和土壤杆菌属ChAg4菌株外，还发现了一个额外的rRNA加工位点。