Tesfaye M, Peterson D J, Holl F B
Department of Plant Science, University of British Columbia, Vancouver, Canada.
Can J Microbiol. 1997 Jun;43(6):526-33. doi: 10.1139/m97-075.
A hypervariable region of Rhizobium 23S rDNA was amplified by polymerase chain reaction and phylogenetic relationships of several strains were determined by comparing nucleotide sequences of the amplified product. Variation in the 23S rDNA nucleotide sequences was consistent with phylogenetic relationships determined by host nodulation specificity and (or) 16S rDNA sequence analysis. Six strains representing three Rhizobium species (R. leguminosarum bv. trifolii, R. meliloti, and R. etli), and two strains each of Bradyrhizobium and Agrobacterium were clustered into five rDNA groups. Unique features identified by secondary structure analysis of the 23S rRNA sequenced region were consistent with the hypothesis that 23S rDNA could be used to design species- or strain-specific Rhizobium probes.
通过聚合酶链反应扩增了根瘤菌23S rDNA的一个高变区，并通过比较扩增产物的核苷酸序列确定了几个菌株的系统发育关系。23S rDNA核苷酸序列的变异与通过宿主结瘤特异性和（或）16S rDNA序列分析确定的系统发育关系一致。代表三种根瘤菌（三叶草根瘤菌、苜蓿根瘤菌和埃氏根瘤菌）的六个菌株，以及慢生根瘤菌和土壤杆菌各两个菌株被聚类为五个rDNA组。通过对23S rRNA测序区域的二级结构分析确定的独特特征与23S rDNA可用于设计根瘤菌属或菌株特异性探针的假设一致。
