Lombardo Y B, Chicco A, D'Alessandro M E, Martinelli M, Soria A, Gutman R
Department of Biochemistry, University of Litoral, Ciudad Universitaria Paraje El Pozo, Santa Fe, Argentina.
Biochim Biophys Acta. 1996 Jan 19;1299(2):175-82. doi: 10.1016/0005-2760(95)00197-2.
The aim of this study was to investigate the relationship between the lipid-lowering effects of fish oils and concomitant consequences on glucose tolerance and insulin sensitivity in an experimental animal model of hypertriglyceridemia induced by high sucrose intake. To achieve this goal, male Wistar rats were fed a semi-synthetic sucrose rich diet (SRD) (w/w: 62.3% sucrose, 8% corn oil, 17% protein) for 90 days. At the time, a well established and permanent hypertriglyceridemia accompanied by glucose intolerance was present. After that, one half of the animals continued on the SRD up to 120 days. The other half received an SRD in which the source of fat was substituted by cod liver oil (w/w 7% CLO plus 1% corn oil) from day 90 to 120 (SRD+CLO). Control rats were fed a semi-synthetic diet (CD) (w/w: 62.5% corn starch, 8% corn oil, 17% protein) throughout the 120 days experimental period. Results obtained after the experimental period show that the hypertriglyceridemia and glucose intolerance ensuing long term feeding normal rats with a sucrose-rich diet could be completely reversed mediating no change in circulating insulin levels by shifting the source of fat in the diet from corn oil to cod liver oil. These findings suggest that manipulation of dietary fats may play a role in the management of the lipid disorders associated with glucose intolerance and insulin resistance.
本研究的目的是在高蔗糖摄入诱导的高甘油三酯血症实验动物模型中，研究鱼油的降血脂作用与对葡萄糖耐量和胰岛素敏感性的伴随影响之间的关系。为实现这一目标，雄性Wistar大鼠被喂食半合成高蔗糖饮食（SRD）（重量比：62.3%蔗糖、8%玉米油、17%蛋白质），持续90天。此时，出现了已确立的持续性高甘油三酯血症并伴有葡萄糖不耐受。之后，一半动物继续食用SRD直至120天。另一半从第90天到120天接受一种脂肪来源被鳕鱼肝油替代的SRD（重量比7%鳕鱼肝油加1%玉米油）（SRD+CLO）。对照大鼠在整个120天的实验期内喂食半合成饮食（CD）（重量比：62.5%玉米淀粉、8%玉米油、17%蛋白质）。实验期结束后获得的结果表明，长期用富含蔗糖的饮食喂养正常大鼠所导致的高甘油三酯血症和葡萄糖不耐受可通过将饮食中的脂肪来源从玉米油改为鳕鱼肝油而完全逆转，且循环胰岛素水平无变化。这些发现表明，饮食脂肪的调控可能在与葡萄糖不耐受和胰岛素抵抗相关的脂质紊乱管理中发挥作用。
