Wollert S, Antonsson J, Gerdin B, Lundberg C, Rasmussen I B, Haglund U
Department of Surgery, University Hospital, Uppsala, Sweden.
Eur J Surg. 1995 Oct;161(10):741-50.
To assess the incidence of intestinal mucosal injury during faecal peritonitis in pigs, the relation of such lesions to haemodynamic variables, intramucosal pH (pHi), and endothelial adherence of polymorphonuclear leucocytes (PMNs).
Experimental laboratory study.
University department of surgery, Sweden.
57 Juvenile pigs.
Pigs had faecal peritonitis induced (n = 39) or a sham procedure (n = 18). In addition, 15 animals were pretreated with the monoclonal CD18 receptor antibody IB4 before induction of peritonitis with the aim of preventing tissue accumulation of PMNs.
Development of mucosal lesions and correlation with haemodynamic variables.
17/18 (94%) of control animals had normal mucosa. The incidence of mucosal lesions in animals with faecal peritonitis was 56%. Animals with severe mucosal injury (grade 4-5) had significantly lower mean arterial pressure, cardiac index, and pHi during the last hour of the experiment compared with animals without mucosal lesions. Pretreatment with IB4 did not prevent the development of intestinal mucosal injuries. Intramucosal pH decreased during sepsis and was not affected by IB4.
Severe intestinal mucosal injury is associated with arterial hypotension, low cardiac index, and low pH. Neither the mucosal injury nor the reduction in pHi seen during porcine faecal peritonitis seemed to be leucocyte-related phenomena.
评估猪粪便性腹膜炎期间肠黏膜损伤的发生率，以及此类损伤与血流动力学变量、黏膜内pH值（pHi）和多形核白细胞（PMN）内皮黏附之间的关系。
实验性实验室研究。
瑞典大学外科系。
57只幼猪。
诱导猪发生粪便性腹膜炎（n = 39）或进行假手术（n = 18）。此外，15只动物在诱导腹膜炎前用单克隆CD18受体抗体IB4进行预处理，目的是防止PMN在组织中积聚。
黏膜损伤的发生情况及其与血流动力学变量的相关性。
18只对照动物中有17只（94%）黏膜正常。粪便性腹膜炎动物的黏膜损伤发生率为56%。与无黏膜损伤的动物相比，严重黏膜损伤（4 - 5级）的动物在实验最后一小时的平均动脉压、心脏指数和pHi显著降低。用IB4预处理并不能预防肠黏膜损伤的发生。脓毒症期间黏膜内pH值下降，且不受IB4影响。
严重的肠黏膜损伤与动脉低血压、低心脏指数和低pH值有关。在猪粪便性腹膜炎期间观察到的黏膜损伤和pHi降低似乎都不是与白细胞相关的现象。
