Fukumatsu Y, Katabuchi H, Okamura H
Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, Japan.
Endocr J. 1995 Aug;42(4):467-73. doi: 10.1507/endocrj.42.467.
The localization of epidermal growth factor (EGF) in the ovary and its effect on proliferation of granulosa cells were investigated in gonadotrophin-primed immature female rats. Immunoreactions with anti-rat EGF monoclonal antibody were observed sparsely in the granulosa layer and antrum of follicles, but not in the theca layer or stromal tissue. The EGF-positive cells were round or oval shaped and often larger than granulosa cells. The localization and morphological appearances of these cells in the follicles were in good agreement with those of macrophages. Although EGF alone did not promote granulosa cell growth in vitro, the labelling index with [3H]thymidine of granulosa cells cultured with 0.1 ng/ml EGF and 0.1 ng/ml basic fibroblast growth factor was significantly greater than that without the growth factors (18.4% vs. 15.8%, P < 0.01). These results suggest that macrophages in follicles may modulate follicular development through a paracrine mechanism by secreting EGF and other growth factors.
在促性腺激素预处理的未成熟雌性大鼠中，研究了表皮生长因子（EGF）在卵巢中的定位及其对颗粒细胞增殖的影响。用抗大鼠EGF单克隆抗体进行免疫反应，在卵泡的颗粒层和卵泡腔中观察到稀疏的反应，但在卵泡膜层或间质组织中未观察到。EGF阳性细胞呈圆形或椭圆形，通常比颗粒细胞大。这些细胞在卵泡中的定位和形态外观与巨噬细胞的定位和形态外观非常一致。虽然单独的EGF在体外不能促进颗粒细胞生长，但与0.1 ng/ml EGF和0.1 ng/ml碱性成纤维细胞生长因子一起培养的颗粒细胞的[3H]胸腺嘧啶核苷标记指数明显高于没有生长因子的情况（18.4%对15.8%，P<0.01）。这些结果表明，卵泡中的巨噬细胞可能通过分泌EGF和其他生长因子，通过旁分泌机制调节卵泡发育。
