Katabuchi H, Fukumatsu Y, Araki M, Suenaga Y, Ohtake H, Okamura H
Department of Obstetrics and Gynecology, Kumamoto University School of Medicine, Japan.
Horm Res. 1996;46 Suppl 1:45-51. doi: 10.1159/000185181.
The effects of macrophages on granulosa cell proliferation were examined using gonadotropin-primed immature female rats and osteopetrotic (op/op) mice, a model defective in monocyte-macrophage lineage cells. Macrophages were found in the follicles at various developmental stages in rats and mice. The labeling index with [3H]thymidine of cultured rat granulosa cells was maximal when they were cultured with peritoneal macrophages at a macrophages:granulosa cell ratio of 0.01. This ratio was similar to those in rat preantral and antral follicles in vivo. In op/op mice, the number of developing follicles was markedly reduced, but increased after daily macrophage-colony-stimulating factor (M-CSF) administration. In the antral follicles of op/op mice, both granulosa cells and macrophages were significantly decreased in number but were increased after M-CSF treatment. Double immunohistochemical staining revealed that epidermal growth factor (EGF)-positive cells were macrophages in the developing rat follicles. These findings suggest that macrophages are located in the developing follicles and participate in promoting granulosa cell growth through a paracrine mechanism by secreting EGF and other cytokines.
利用促性腺激素预处理的未成熟雌性大鼠和骨硬化症（op/op）小鼠（一种单核细胞-巨噬细胞谱系细胞缺陷模型），研究了巨噬细胞对颗粒细胞增殖的影响。在大鼠和小鼠不同发育阶段的卵泡中均发现了巨噬细胞。当培养的大鼠颗粒细胞与腹腔巨噬细胞以巨噬细胞：颗粒细胞比例为0.01共同培养时，其[3H]胸腺嘧啶核苷标记指数最高。该比例与体内大鼠窦前卵泡和窦卵泡中的比例相似。在op/op小鼠中，发育中的卵泡数量明显减少，但在每日给予巨噬细胞集落刺激因子（M-CSF）后增加。在op/op小鼠的窦卵泡中，颗粒细胞和巨噬细胞的数量均显著减少，但在M-CSF处理后增加。双重免疫组化染色显示，在发育中的大鼠卵泡中，表皮生长因子（EGF）阳性细胞为巨噬细胞。这些发现表明，巨噬细胞位于发育中的卵泡中，并通过分泌EGF和其他细胞因子以旁分泌机制参与促进颗粒细胞生长。
