Chan K C, Muschik G M, Issaq H J
SAIC Frederick, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702, USA.
J Chromatogr A. 1995 Dec 1;718(1):203-10. doi: 10.1016/0021-9673(95)00675-3.
Micellar electrokinetic chromatography (MEKC) was applied for the separation of tryptophan and related indoles. Using a 5 mM sodium borate buffer (pH 9.2) containing 50 mM sodium dodecyl sulfate and 5% acetonitrile, eleven indoles were baseline separated in under 17 min. Most of the indoles were detected at the nM level by native fluorescence using KrF laser-induced fluorescence (LIF), which was approximately 100 times more sensitive than UV absorption detection at 200 nm. Preliminary results show that the MEKC-LIF with direct sample injection is a feasible method for assessing indole profiles in diluted urine and serum.
