Imamura T, Konishi K
Department of Biophysical Chemistry, Dokkyo University School of Medicine, Tochigi, Japan.
J Protein Chem. 1995 Aug;14(6):409-17. doi: 10.1007/BF01888135.
The distribution of indole and tryptophan derivatives between sodium dodecyl sulfate (SDS) micellar and aqueous phases was analyzed using conventional methods of ultraviolet (UV) absorption spectroscopy and measurement of fluorescence quenching by succinimide. On the assumption of a simple pseudo-phase equilibrium between both phases the distribution coefficient was easily obtained by the measurement of the ratio Rpv of the absorbance intensity in the peak to that in the valley of the UV spectra or the fluorescence quenching constant Ksv. The possibilities and limitations of utilizing the ratio of the collisional quenching constant estimating from the Ksv value in the micellar phase to that in the aqueous phase for a measure of polarity of the microenvironment around the tryptophan derivatives in the SDS micelle is discussed in comparison with the Rpv values for the UV spectra. The indole ring in the derivatives in the SDS micelle is localized near or on the micelle-water interface with its imino group directed toward the aqueous phase. Thus it can serve as a feasible model for interpreting the distribution coefficients and Rpv values obtained for the various indole and tryptophan derivatives.
使用传统的紫外（UV）吸收光谱法以及琥珀酰亚胺荧光猝灭测量法，分析了十二烷基硫酸钠（SDS）胶束相和水相之间吲哚和色氨酸衍生物的分布情况。基于两相之间简单的假相平衡假设，通过测量紫外光谱峰谷处吸光度强度之比Rpv或荧光猝灭常数Ksv，可轻松获得分配系数。与紫外光谱的Rpv值相比，讨论了利用胶束相中由Ksv值估算的碰撞猝灭常数与水相中碰撞猝灭常数之比来衡量SDS胶束中色氨酸衍生物周围微环境极性的可能性和局限性。SDS胶束中衍生物的吲哚环位于胶束 - 水界面附近或其上，其亚氨基指向水相。因此，它可以作为一个可行的模型，用于解释各种吲哚和色氨酸衍生物的分配系数和Rpv值。
