Jung W I, Widmaier S, Seeger U, Bunse M, Staubert A, Sieverding L, Straubinger K, van Erckelens F, Schick F, Dietze G, Lutz O
Hypertension and Diabetes Research Unit, Max Grundig Clinic, Bühl, Germany.
J Magn Reson B. 1996 Jan;110(1):39-46. doi: 10.1006/jmrb.1996.0005.
Proton-decoupled 31P NMR spectroscopy of the heart and calf muscle of healthy volunteers was performed with a 1.5 T whole-body imager. By use of two-dimensional chemical-shift imaging in combination with slice-selective excitation, well-resolved localized spectra (elements of 38 ml) were obtained within 20 to 35 min from which the homonuclear J coupling constants of ATP could be determined. In myocardium, J gamma beta = 16.03 +/- 0.17 Hz and J alpha beta = 15.82 +/- 0.23 Hz were obtained, while the values in calf muscle were J gamma beta = 17.16 +/- 0.12 Hz and J alpha beta = 16.04 +/- 0.09 Hz. The difference in J gamma beta was significant. According to the literature, a possible reason for greater ATP J coupling constants is a smaller fraction of ATP complexed to magnesium. However, the chemical-shift difference between alpha- and beta-ATP, which is also a measure for the fraction of ATP complexed to magnesium, showed only a small difference in ATP complexation: 88% in myocardium and 90% in calf muscle. This small difference cannot account for the observed difference in J gamma beta.
利用1.5T全身成像仪对健康志愿者的心脏和小腿肌肉进行了质子去耦31P核磁共振波谱分析。通过二维化学位移成像结合切片选择性激发，在20至35分钟内获得了分辨率良好的局部光谱（38毫升的单元），从中可以确定ATP的同核J耦合常数。在心肌中，Jγβ = 16.03±0.17Hz，Jαβ = 15.82±0.23Hz，而小腿肌肉中的值为Jγβ = 17.16±0.12Hz，Jαβ = 16.04±0.09Hz。Jγβ的差异具有显著性。根据文献，ATP耦合常数较大的一个可能原因是与镁络合的ATP比例较小。然而，α-ATP和β-ATP之间的化学位移差异（也是衡量与镁络合的ATP比例的指标）在ATP络合方面仅显示出很小的差异：心肌中为88%，小腿肌肉中为90%。这种微小差异无法解释观察到的Jγβ差异。
