使用冷冻保存的单核白细胞进行疫苗试验的人类抗病毒细胞毒性T淋巴细胞反应分析：流感病毒特异性反应的可行性证明
Analysis of human antiviral cytotoxic T-lymphocyte responses for vaccine trials using cryopreserved mononuclear leukocytes: demonstration of feasibility with influenza virus-specific responses.
作者信息
el-Daher N, Nichols J E, Roberts N J
机构信息
Department of Medicine, University of Rochester School of Medicine, New York 14642, USA.
出版信息
Clin Diagn Lab Immunol. 1994 Sep;1(5):487-92. doi: 10.1128/cdli.1.5.487-492.1994.
Abstract
The feasibility of measuring virus-specific human cytotoxic T-lymphocyte (CTL) activity by using cryopreserved mononuclear leukocytes to support clinical vaccine trials was addressed. Autologous fresh and cryopreserved cells from the same sample of peripheral blood were used as sources of CTL precursors and were tested for influenza virus-specific activity. The data indicated that virus-specific CTL activity could be measured by using cryopreserved cells; this could also be done in assays that are designed to characterize the responsible effector cell population.
摘要
探讨了使用冷冻保存的单核白细胞来测定病毒特异性人类细胞毒性T淋巴细胞（CTL）活性以支持临床疫苗试验的可行性。来自同一份外周血样本的自体新鲜细胞和冷冻保存细胞用作CTL前体来源，并检测其流感病毒特异性活性。数据表明，可使用冷冻保存的细胞来测定病毒特异性CTL活性；这也可以在旨在鉴定相关效应细胞群体的检测中实现。
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