de Souza Queiroz L, de Paula Eduardo R M
Acta Neuropathol. 1977 Apr 29;38(1):45-8. doi: 10.1007/BF00691275.
In order to ascertain the immediate histopathological changes of rat brain following experimental surgical injury, fragments of left parietal cortex were obtained through craniotomy and fixed by immersion. Next, the animals were killed by perfusion with the same fixative used for the respective biopsy. Five groups of rats were tested, each for one different fixative. Dark neurons were by far the most prominent feature in surgically traumatized tissue, following both immersion and perfusion with all five fluids. They were morphologically identical at both sites, and fixatives with widely different chemical composition, e.g. Bouin's fluid and buffered glutaraldehyde, had no influence on their aspect. It is suggested that shrunken neurons are not to be interpreted systematically as artefacts, but also represent a form of short-latency cellular reaction to injury.
为了确定实验性手术损伤后大鼠脑的即时组织病理学变化，通过开颅术获取左顶叶皮质碎片并进行浸液固定。接下来，用用于各自活检的相同固定剂灌注处死动物。测试了五组大鼠，每组使用一种不同的固定剂。在使用所有五种液体进行浸液固定和灌注后，深色神经元是手术创伤组织中最显著的特征。它们在两个部位的形态相同，化学组成差异很大的固定剂，如波因氏液和缓冲戊二醛，对其外观没有影响。有人提出，萎缩的神经元不应一概被视为人为假象，而也代表了对损伤的一种短潜伏期细胞反应形式。
