Li H, Liu J, Hofmann M
Division of Molecular Biology, Shenyang Medical College.
Zhonghua Yi Xue Za Zhi. 1995 Sep;75(9):525-8, 573.
We investigated CD44 expression patterns in five types of primary brain tumors and brain metastases from more than ten organs by Northern blot, reverse transcription/polymerase chain reaction (RT/PCR) and immunocytochemical analyses. The results demonstrated that all of 72 primary brain tumors examined express standard form of CD44 (CD44s) but none of them express CD44v. In contrast, 22 of 26 brain metastases studied were found to express CD44v. Our data not only present a direct evidence of a general distribution of CD44 in primary brain tumors but also suggest that such expression is an extremely rare event in primary brain tumors. We conclude that CD44v may be a candidate marker for the evaluation of malignant potential of the tumors and the prognosis of cancer patients.
我们通过Northern印迹法、逆转录/聚合酶链反应（RT/PCR）和免疫细胞化学分析，研究了五种原发性脑肿瘤以及来自十多个器官的脑转移瘤中的CD44表达模式。结果表明，所检测的72例原发性脑肿瘤均表达标准形式的CD44（CD44s），但均不表达CD44v。相比之下，在研究的26例脑转移瘤中，有22例被发现表达CD44v。我们的数据不仅直接证明了CD44在原发性脑肿瘤中的普遍分布，还表明这种表达在原发性脑肿瘤中是极为罕见的事件。我们得出结论，CD44v可能是评估肿瘤恶性潜能和癌症患者预后的候选标志物。
