Webster R P, Gawde M D, Bhattacharya R K
Radiation Biology and Biochemistry Division, Bhabha Atomic Research Centre, Bombay, India.
Cancer Lett. 1996 Jan 2;98(2):129-35.
Formation of single strand breaks in nuclear DNA induced by hepatocarcinogens aflatoxin B1 and N-nitrosodimethylamine was observed to be more pronounced in rats maintained on a riboflavin-deficient diet compared to that on a normal diet. This increased damage was reversed on riboflavin supplementation. The induction of repair enzymes poly(ADP-ribose) polymerase, DNA polymerase beta and DNA ligase was significantly higher in riboflavin-deficient rats following DNA damage caused by the administration of carcinogens. Riboflavin supplementation brought down the induction to the levels found in rats maintained on normal diet. Since damage to DNA and its altered repair may relate to carcinogenesis, modulation of these parameters by riboflavin suggests a potential chemopreventive role of this vitamin.
观察发现，与正常饮食的大鼠相比，食用核黄素缺乏饮食的大鼠，由肝癌致癌物黄曲霉毒素B1和N-亚硝基二甲胺诱导的核DNA单链断裂更为明显。补充核黄素后，这种增加的损伤得到了逆转。在给予致癌物导致DNA损伤后，核黄素缺乏的大鼠中修复酶聚（ADP-核糖）聚合酶、DNA聚合酶β和DNA连接酶的诱导水平显著更高。补充核黄素使诱导水平降至正常饮食大鼠的水平。由于DNA损伤及其改变的修复可能与致癌作用有关，核黄素对这些参数的调节表明这种维生素具有潜在的化学预防作用。
