Campos L, Roubi N, Guyotat D
Centre de Transfusion Sanguine, Lyon, France.
Cryobiology. 1995 Dec;32(6):511-5. doi: 10.1006/cryo.1995.1052.
Prior studies have shown that human umbilical cord blood cells can restore hematopoiesis and be used as a source of stem cells for hematopoietic transplantation. We have performed a study of the best conditions of collection and cryopreservation of blood from eight umbilical cords. We compared the influence of cell separation and of delay between collection and cryopreservation on the numbers of nucleated cells and of hematopoietic progenitors recovered before and after cryopreservation. Ficoll separation resulted in the loss of more than 50% of nucleated cells, but also of a significant number of progenitors before freezing. Unseparated cells could be kept at 25 degrees C as long as 24 h before freezing with minimal loss of progenitors before and after freezing and thawing. In contrast, there was a significant decrease in the number of viable cells and progenitors when cells were maintained at 4 degrees C before freezing. Our data show that cord blood banking is feasible with simple collection and cryopreservation procedures.
先前的研究表明，人脐带血细胞能够恢复造血功能，并可作为造血移植的干细胞来源。我们对取自八条脐带的血液进行了采集和冷冻保存最佳条件的研究。我们比较了细胞分离以及采集与冷冻保存之间的延迟对冷冻保存前后回收的有核细胞和造血祖细胞数量的影响。Ficoll分离导致超过50%的有核细胞丢失，同时在冷冻前也有大量祖细胞丢失。未分离的细胞在冷冻前可在25摄氏度下保存长达24小时，冷冻和解冻前后祖细胞的损失最小。相比之下，在冷冻前将细胞保存在4摄氏度时，活细胞和祖细胞的数量会显著减少。我们的数据表明，通过简单的采集和冷冻保存程序进行脐带血库储存是可行的。
