细胞库操作对脐带血体外扩增的影响。

Effects of cell banking manipulations on ex vivo amplification of umbilical cord blood.

作者信息

Forte L, Migliaccio G, Sanchez M, Migliaccio A R, Passarelli A M, Amadori S

机构信息

Dipartimento di Ematologia, Università degli Studi Tor Vergata, Rome, Italy.

出版信息

Ann Ist Super Sanita. 2000;36(3):333-42.

PMID:11293302

Abstract

The small volume of placental/umbilical cord blood (PUCB) collectable restricts the use of these stem cells to pediatric transplantation. To extend the use of PUCB to adult recipients, many laboratories are investigating the feasibility of ex vivo PUCB expansion. The present study analyses the effects that PUCB banking cell manipulations (cell sedimentation, cryopreservation and thawing, mononuclear and CD34+ cell isolation) have on the number, viability and ex vivo expansion potential of PUCB cells. The results presented indicate the necessity of an open discussion on whether procedures used for handling the cells in PUCB banks can be extrapolated or not as such to the clinical use of ex vivo expanded PUCB.

摘要

可采集的胎盘/脐带血（PUCB）量较少，这限制了这些干细胞仅用于儿科移植。为了将PUCB的使用扩展到成年受者，许多实验室正在研究体外PUCB扩增的可行性。本研究分析了PUCB库细胞操作（细胞沉降、冷冻保存和解冻、单核细胞和CD34+细胞分离）对PUCB细胞数量、活力和体外扩增潜力的影响。所呈现的结果表明，有必要就是否可以将PUCB库中处理细胞的程序直接类推用于体外扩增PUCB的临床应用展开公开讨论。