Ruiz P, Geraldino N
Department of Pathology, University of Miami School of Medicine, Florida.
Cytometry. 1995 Sep 15;22(3):211-6. doi: 10.1002/cyto.990220308.
Previous studies have suggested that gamma delta T cells can be increased in HIV-1-seropositive individuals, although characterization of gamma delta T cell subtypes and correlation with clinical status of these patients have not been performed. We investigated groups of HIV-seropositive persons to determine the prevalence of elevated levels of gamma delta T cells and whether any gamma delta T cell subtypes were preferentially expressed. Since a large proportion of human gamma delta T cells appear to be reactive to proteins encoded by mycobacteria, we also examined our patients for the incidence of mycobacterial infection. Our results show that a significant number of HIV-positive patients have an elevated number of gamma delta T cells in their peripheral blood as compared to normal controls. HIV-seropositive patients with clinical or laboratory evidence of mycobacterial infection had statistically significant increases in the percentage and total numbers of gamma delta T cells over the HIV-positive persons without mycobacterial infection. An examination of the subtypes of gamma delta T cells revealed that certain subtypes such as V gamma 9+ and V delta 2+ T cells were preferentially elevated in the mycobacteria-positive patients. These results suggest that an increased number of gamma delta T cells in HIV-positive patients is most often seen in the setting of an opportunistic mycobacterial infection and that specific gamma delta T cell subtypes are stimulated under these conditions. The role of these increased number of gamma delta T cells in HIV-associated disease is unclear but is likely a component of the response and degree of host resistance to this organism.
以往研究表明，在HIV-1血清反应阳性个体中γδT细胞数量可能增加，尽管尚未对γδT细胞亚型进行特征描述，也未研究这些患者的γδT细胞数量与临床状态的相关性。我们对HIV血清反应阳性人群进行了调查，以确定γδT细胞水平升高的患病率，以及是否有任何γδT细胞亚型被优先表达。由于很大一部分人类γδT细胞似乎对分枝杆菌编码的蛋白质有反应，我们还检查了患者的分枝杆菌感染发生率。我们的结果显示，与正常对照组相比，大量HIV阳性患者外周血中的γδT细胞数量增加。有分枝杆菌感染临床或实验室证据的HIV血清反应阳性患者，其γδT细胞百分比和总数在统计学上显著高于无分枝杆菌感染的HIV阳性患者。对γδT细胞亚型的检查发现，某些亚型如Vγ9+和Vδ2+T细胞在分枝杆菌阳性患者中优先升高。这些结果表明，HIV阳性患者中γδT细胞数量增加最常见于机会性分枝杆菌感染的情况下，并且在这些条件下特定的γδT细胞亚型会受到刺激。这些数量增加的γδT细胞在HIV相关疾病中的作用尚不清楚，但可能是宿主对该病原体反应和抵抗程度的一个组成部分。
