Agostini C, Zambello R, Trentin L, Cerutti A, Bulian P, Crivellaro C, Cipriani A, Semenzato G
Department of Clinical Medicine, Padua University School of Medicine, Italy.
Cell Immunol. 1994 Jan;153(1):194-205. doi: 10.1006/cimm.1994.1017.
In this study the frequency of gamma delta+ cells and their subsets has been assessed in bronchoalveolar lavage (BAL) cell populations recovered from 51 patients at various clinical stages of HIV-1 infection. Thirteen out of the 51 HIV-1-infected patients showed an increase in the percentage of TCR delta 1+ BAL T cells (25.5%). BAL lymphocytes bearing pan-gamma delta antigens were also quantitatively increased in 10 patients (19.6%). A strict correlation was observed between the degree of CD8 alveolitis and the increase of gamma delta T cells. Phenotypic study of BAL gamma delta cells revealed that (a) V delta 2-related BB3+ cells accounted for the majority of lung gamma delta T cells; (b) these cells were CD45RO+ memory cells and expressed a series of adhesion molecules; and (c) 29% of BAL gamma delta T cells expressed CD8 surface molecules. We also compared the distribution of V delta 2 and V delta 1 subsets in paired samples of peripheral blood and BAL fluid. Patients who showed an increased number of BB3+ cells in the BAL fluid presented a reversal of the V delta 2 to V delta 1 cell ratio in the peripheral blood. By contrast, in the lung of normal subjects pulmonary BB3+ and A13+ cells were present in approximately the same proportions found in the peripheral blood. Taken together these data demonstrate that a redistribution of T cells expressing V delta 2 TCR takes place in the lung of a subset of patients with HIV-1 infection and CD8 alveolitis. In the pulmonary microenvironment these cells might play a role in the local immune response against HIV-1 and/or opportunistic infections.
在本研究中，我们评估了从51例处于不同临床阶段的HIV-1感染患者中回收的支气管肺泡灌洗（BAL）细胞群体中γδ+细胞及其亚群的频率。51例HIV-1感染患者中有13例显示TCRδ1+BAL T细胞百分比增加（25.5%）。10例患者（19.6%）中携带泛γδ抗原的BAL淋巴细胞数量也有定量增加。观察到CD8肺泡炎程度与γδT细胞增加之间存在密切相关性。对BALγδ细胞的表型研究表明：（a）Vδ2相关的BB3+细胞占肺γδT细胞的大多数；（b）这些细胞是CD45RO+记忆细胞，并表达一系列粘附分子；（c）29%的BALγδT细胞表达CD8表面分子。我们还比较了外周血和BAL液配对样本中Vδ2和Vδ1亚群的分布。BAL液中BB3+细胞数量增加的患者外周血中Vδ2与Vδ1细胞比例发生了逆转。相比之下，在正常受试者的肺中，肺BB3+和A13+细胞的比例与外周血中大致相同。综合这些数据表明，在一部分HIV-1感染和CD8肺泡炎患者的肺中，表达Vδ2 TCR的T细胞发生了重新分布。在肺微环境中，这些细胞可能在针对HIV-1和/或机会性感染的局部免疫反应中发挥作用。
