Tabernero M D, San Miguel J F, Garcia J L, Garcia-Isidoro M, Wiegant J, Ciudad J, Gonzalez M, Rios A, Raap A, Orfao A
Unit of Cytometry, University of Salamanca, Spain.
Cytometry. 1995 Sep 15;22(3):217-22. doi: 10.1002/cyto.990220309.
The clinical, biological, and immunophenotypical characteristics of B-cell chronic lymphocytic leukemia (B-CLL) patients with trisomy 12 detected by fluorescence in situ hybridization (FISH) using a chromosome 12 alpha-centromeric probe (D12Z3) were analyzed in the present study. From a total of 104 consecutive B-CLL patients, 21 (20%) displayed trisomy 12, the percentage of trisomic cells ranging from 13% to 76%. From the clinico-biological point of view, patients with trisomy 12 were associated with atypical CLL morphology (43% vs 10%, P = 0.04) and BM diffuse pattern (75% vs. 25%, P = 0.02) together with increased WBC counts (141 +/- 220 vs. 58 +/- 67 x 10(9)/L, P = 0.04). In contrast, no association was detected between the presence of trisomy 12 and other disease characteristics such as age, sex, clinical stage, hepatomegaly, lymphadenopathies, haemoglobin levels and platelet counts, and the cell cycle distribution of PB leukocytes in both groups of patients. Trisomy 12 patients had a significantly higher expression of the FMC7 antigen both in percentage (34 +/- 34% vs. 13 +/- 20%, P = 0.02) and absolute numbers (29 +/- 62 vs. 7 +/- 17 x 10(9)/L, P = 0.007). No major differences were found regarding the expression of mouse rosettes, CD19+, and CD19+/CD5+ lymphocytes. Upon analyzing the correlations between the disease characteristics of trisomy 12 cases, significant associations were found between the percentage of trisomic cells and both the WBC count (r = 0.52, P = 0.02) and the PB lymphocyte count (r = 0.60, P = 0.007).(ABSTRACT TRUNCATED AT 250 WORDS)
本研究分析了使用12号染色体α着丝粒探针（D12Z3）通过荧光原位杂交（FISH）检测到的12号染色体三体的B细胞慢性淋巴细胞白血病（B-CLL）患者的临床、生物学和免疫表型特征。在104例连续的B-CLL患者中，21例（20%）显示12号染色体三体，三体细胞百分比范围为13%至76%。从临床生物学角度来看，12号染色体三体的患者与非典型CLL形态（43%对10%，P = 0.04）和骨髓弥漫模式（75%对25%，P = 0.02）相关，同时白细胞计数增加（141±220对58±67×10⁹/L，P = 0.04）。相比之下，在两组患者中，未检测到12号染色体三体的存在与其他疾病特征如年龄、性别、临床分期、肝肿大、淋巴结病、血红蛋白水平和血小板计数以及外周血白细胞的细胞周期分布之间存在关联。12号染色体三体的患者FMC7抗原的表达在百分比（34±34%对13±20%，P = 0.02）和绝对数量（29±62对7±17×10⁹/L，P = 0.007）上均显著更高。在小鼠玫瑰花结、CD19⁺和CD19⁺/CD5⁺淋巴细胞的表达方面未发现重大差异。在分析12号染色体三体病例的疾病特征之间的相关性时，发现三体细胞百分比与白细胞计数（r = 0.52，P = 0.02）和外周血淋巴细胞计数（r = 0.60，P = 0.007）之间均存在显著关联。（摘要截断于250字）
