从单个蜱虫卵、幼虫和若虫中进行诊断性DNA扩增。
Diagnostic DNA amplification from individual tick eggs, larvae and nymphs.
作者信息
Zahler M, Gothe R, Rinder H
机构信息
Institute for Comparative Tropical Medicine and Parasitology, University of Munich, Germany.
出版信息
Exp Appl Acarol. 1995 Dec;19(12):731-6. doi: 10.1007/BF00052084.
The species of single tick eggs, larvae and nymphs was determined by PCR amplification and characterization of the hypervariable, second transcribed spacer (ITS2) of the multicopy ribosomal RNA gene (rDNA). Engorgement of larvae and nymphs did not preclude species identification. The method is generally applicable for ixodid and argasid ticks and can be used for epidemiological studies requiring the identification of individuals from pre-adult stages.
通过对多拷贝核糖体RNA基因（rDNA）的高变第二转录间隔区（ITS2）进行PCR扩增和特征分析，确定单只蜱虫卵、幼虫和若虫的种类。幼虫和若虫饱血并不妨碍种类鉴定。该方法一般适用于硬蜱和软蜱，可用于需要鉴定未成熟阶段个体的流行病学研究。
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