Colleu D, Lescoat D, Gouranton J
Center for Infertility and Assisted Reproductive Technology La Sagesse, University of Rennes, France.
Fertil Steril. 1996 Jan;65(1):160-4. doi: 10.1016/s0015-0282(16)58045-5.
To investigate the relationship between sperm preparation techniques and nuclear maturity, as evidenced by the electrophoretic profiles of sperm nuclear proteins.
Analysis of sperm nuclear quality in sperm populations used for IVF.
Center for infertility and assisted reproductive technology and university departments.
Twenty-seven men undergoing an infertility work-up.
Comparative electrophoretic investigation of nucleoproteins extracted from spermatozoa selected by swim-up or Percoll techniques.
Nuclear maturity level is improved after the two methods of selection but is more improved after Percoll. In the two groups, selected spermatozoa contain less histones. Moreover, Percoll gradients appeared to enrich for spermatozoa with less intermediate proteins and more mature nucleoproteins of P2 family than swim-up spermatozoa.
Percoll may offer advantages in terms of the quality of the selected spermatozoa that may influence the outcome of assisted conception techniques.
通过精子核蛋白的电泳图谱来研究精子制备技术与核成熟度之间的关系。
对用于体外受精的精子群体的精子核质量进行分析。
不孕不育与辅助生殖技术中心及大学各系。
27名接受不孕症检查的男性。
对通过上浮法或Percoll技术选择的精子中提取的核蛋白进行比较电泳研究。
两种选择方法后核成熟度水平均有所提高，但Percoll法提高得更多。在两组中，所选精子含有的组蛋白较少。此外，与上浮法选择的精子相比，Percoll梯度似乎能富集更多中间蛋白较少、P2家族成熟核蛋白较多的精子。
就所选精子的质量而言，Percoll可能具有优势，这可能会影响辅助受孕技术的结果。
