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A Simple, Centrifugation-Free, Sperm-Sorting Device Eliminates the Risks of Centrifugation in the Swim-Up Method While Maintaining Functional Competence and DNA Integrity of Selected Spermatozoa.一种简单的、无需离心的精子分选装置，在保持所选精子功能完整性和 DNA 完整性的同时，消除了上游法中离心的风险。
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本文引用的文献

1
Association between the extent of DNA damage in the spermatozoa, fertilization and developmental competence in preimplantation stage embryos.精子中DNA损伤程度与植入前阶段胚胎的受精及发育能力之间的关联。
J Turk Ger Gynecol Assoc. 2010 Dec 1;11(4):182-6. doi: 10.5152/jtgga.2010.34. eCollection 2010.
2
The comet assay as an indicator test for germ cell genotoxicity.彗星试验作为生殖细胞遗传毒性的指示性检测方法。
Mutat Res. 2009 Jan-Feb;681(1):3-12. doi: 10.1016/j.mrrev.2008.03.005. Epub 2008 Mar 30.
3
Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique.精液制备方法与精子凋亡：上游法与梯度密度离心技术
Fertil Steril. 2009 Feb;91(2):632-8. doi: 10.1016/j.fertnstert.2007.11.068. Epub 2008 Jan 18.
4
Origins and consequences of DNA damage in male germ cells.男性生殖细胞中DNA损伤的起源与后果。
Reprod Biomed Online. 2007 Jun;14(6):727-33. doi: 10.1016/s1472-6483(10)60676-1.
5
Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates.通过密度梯度离心法进行精液处理并不能提高精子凋亡性脱氧核糖核酸片段化率。
Fertil Steril. 2008 Sep;90(3):889-90. doi: 10.1016/j.fertnstert.2007.01.059. Epub 2007 May 4.
6
p21 provides stage specific DNA damage control to preimplantation embryos.p21为植入前胚胎提供阶段特异性的DNA损伤控制。
Oncogene. 2007 Sep 13;26(42):6141-9. doi: 10.1038/sj.onc.1210444. Epub 2007 Apr 9.
7
Evaluation of DNA damage in different stages of mouse spermatogenesis after testicular X irradiation.睾丸X线照射后小鼠精子发生不同阶段的DNA损伤评估。
Radiat Res. 2003 Oct;160(4):443-51. doi: 10.1667/rr3053.
8
Germ cell and dose-dependent DNA damage measured by the comet assay in murine spermatozoaa after testicular X-irradiation.睾丸X线照射后，通过彗星试验检测小鼠精子中的生殖细胞和剂量依赖性DNA损伤。
Biol Reprod. 2002 Sep;67(3):854-61. doi: 10.1095/biolreprod.102.004382.
9
Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques.精子染色质结构分析：其在男性不育症中检测精子DNA碎片化的临床应用及与其他技术的比较
J Androl. 2002 Jan-Feb;23(1):25-43. doi: 10.1002/j.1939-4640.2002.tb02599.x.
10
Comparison of sperm preparation methods: effect on chromatin and morphology recovery rates and their consequences on the clinical outcome after in vitro fertilization embryo transfer.精子制备方法的比较：对染色质和形态恢复率的影响及其对体外受精胚胎移植后临床结局的影响
Int J Androl. 2001 Dec;24(6):360-8. doi: 10.1046/j.1365-2605.2001.0317a.x.

上浮法和密度梯度分离法相结合可有效去除DNA损伤的精子。

Combination of swim-up and density gradient separation methods effectively eliminate DNA damaged sperm.

作者信息

Ghumman Surveen, Adiga Satish Kumar, Upadhya Dinesh, Kalthur Guruprasad, Jayaraman Varshini, Rao Satish Bola, Kumar Pratap

机构信息

Clinical Embryology, Division of Reproductive Medicine, Kasturba Medical College, Manipal University, Manipal, India.

Department of Radiobiology and Toxicology, Manipal Life Science Centre, Manipal, India.

出版信息

J Turk Ger Gynecol Assoc. 2011 Sep 1;12(3):148-52. doi: 10.5152/jtgga.2011.35. eCollection 2011.

DOI:10.5152/jtgga.2011.35
PMID:24591982
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3939271/
Abstract

OBJECTIVE

The aim of this experimental prospective study was to investigate the efficacy of single and combination sperm wash methods for their ability to isolate DNA intact spermatozoa.

MATERIAL AND METHODS

Sperm DNA damage was introduced by local testicular irradiation in male mice and the extent of damage was quantified by comet assay. The spermatozoa were subjected to single (swim up or density gradient method) and also a combination of sperm wash techniques. The DNA integrity in various sub-fractions of wash techniques was evaluated.

RESULTS

The amount of DNA damaged sperm did not differ between individual fractions when single wash technique was applied. However, a combination of density gradient and swim-up techniques significantly reduced (p<0.01) the number of DNA damaged sperm in the final population.

CONCLUSION

The combination of density gradient separation and swim-up method is effective in eliminating DNA damaged spermatozoa.

摘要

目的

本实验性前瞻性研究的目的是调查单一及联合精子洗涤方法分离DNA完整精子的能力。

材料与方法

通过对雄性小鼠进行局部睾丸照射引入精子DNA损伤，并通过彗星试验对损伤程度进行量化。对精子采用单一方法（上游法或密度梯度法）以及联合精子洗涤技术进行处理。评估洗涤技术各亚组分中的DNA完整性。

结果

采用单一洗涤技术时，各组分中DNA受损精子的数量没有差异。然而，密度梯度法和上游法联合使用可显著减少（p<0.01）最终群体中DNA受损精子的数量。

结论

密度梯度分离法和上游法联合使用可有效去除DNA受损精子。