Sofikitis N V, Toda T, Miyagawa I, Zavos P M, Pasyianos P, Mastelou E
Tottori University School of Medicine, Yonago, Japan.
Fertil Steril. 1996 Jan;65(1):176-85. doi: 10.1016/s0015-0282(16)58048-0.
To evaluate the effects of electrical stimulation of rabbit oocytes before round spermatid nuclear injection procedure on oocyte activation and fertilization.
The ratio of activated oocytes to the number of successfully injected oocytes and the proportion of offspring to the number of activated oocytes after round spermatid nuclear injections into oocytes stimulated via mechanical stimulation (group A) or a combination of electrical and mechanical stimulation (group B) was compared.
Round spermatid nuclei were isolated from mature male rabbits and microinjected into the oocytes of groups A and B. Injected oocytes were cultured for 24 hours. The embryos developed from groups A and B were transferred to synchronized recipient does.
Embryos that developed normally through implantation in groups A and B were carried successfully through complete gestation in the recipient does. The ratio of the activated oocytes to the number of successfully injected oocytes and the proportion of offspring to the number of activated oocytes were significantly higher in group B.
Electrical stimulation of oocytes before ooplasmic spermatid nuclear injections and ET procedures has beneficial effects on oocytes activation, fertilization, and subsequent embryonic development.
评估在圆形精子细胞核注射程序之前对兔卵母细胞进行电刺激对卵母细胞激活和受精的影响。
比较通过机械刺激（A组）或电刺激与机械刺激联合（B组）刺激后的卵母细胞，将圆形精子细胞核注入其中后，激活的卵母细胞与成功注射的卵母细胞数量之比，以及激活的卵母细胞产生后代的比例。
从成年雄性兔中分离出圆形精子细胞核，并显微注射到A组和B组的卵母细胞中。将注射后的卵母细胞培养24小时。将A组和B组发育的胚胎移植到同期受体母兔体内。
A组和B组中通过植入正常发育的胚胎在受体母兔体内成功完成整个妊娠期。B组中激活的卵母细胞与成功注射的卵母细胞数量之比以及激活的卵母细胞产生后代的比例显著更高。
在卵细胞质内精子细胞核注射和胚胎移植程序之前对卵母细胞进行电刺激，对卵母细胞激活、受精及随后的胚胎发育具有有益影响。
