卵胞浆内圆形精子细胞注射程序作为非梗阻性无精子症的一种实验性治疗方法。
Ooplasmic round spermatid nuclear injection procedures as an experimental treatment for nonobstructive azoospermia.
作者信息
Yamanaka K, Sofikitis N V, Miyagawa I, Yamamoto Y, Toda T, Antypas S, Dimitriadis D, Takenaka M, Taniguchi K, Takahashi K, Tsukamoto S, Kawamura H, Neil M
机构信息
Department of Urology, Tottori University School of Medicine, Yonago, Japan.
出版信息
J Assist Reprod Genet. 1997 Jan;14(1):55-62. doi: 10.1007/BF02765754.
PURPOSE
Our objective was to apply ooplasmic round spermatid nuclear injections for the treatment of nonobstructive azoospermia.
MATERIALS
Participants were nine azoospermic men who had previously undergone diagnostic testicular biopsy. Spermatogenetic arrest was diagnosed at the round spermatid stage (n = 6) or primary spermatocyte stage (n = 3). A second (therapeutic) testicular biopsy was performed and round spermatid nuclei were recovered from all the participants.
RESULTS
Forty-nine mature oocytes were successfully injected with nuclei and then cultured for 72 hr. Twenty-four embryos were transferred to nine women. No pregnancy was achieved.
CONCLUSIONS
Round spermatids can be recovered from therapeutic testicular biopsy material of men negative for round spermatids in previous routine diagnostic testicular biopsy specimens. Round spermatid nuclear injections may play a role in the treatment of nonobstructive azoospermia.
目的
我们的目标是应用卵胞质内圆形精子细胞核注射技术治疗非梗阻性无精子症。
材料
参与者为9名曾接受诊断性睾丸活检的无精子症男性。精子发生阻滞诊断为圆形精子细胞期（n = 6）或初级精母细胞期（n = 3）。进行了第二次（治疗性）睾丸活检，并从所有参与者中获取了圆形精子细胞核。
结果
49个成熟卵母细胞成功进行了细胞核注射，然后培养72小时。24个胚胎被移植到9名女性体内。未实现妊娠。
结论
在先前常规诊断性睾丸活检标本中圆形精子细胞为阴性的男性的治疗性睾丸活检材料中可以获取圆形精子细胞。圆形精子细胞核注射可能在非梗阻性无精子症的治疗中发挥作用。
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