McKeand J B, Kennedy M W
Department of Veterinary Parasitology, University of Glasgow, UK.
Int J Parasitol. 1995 Oct;25(10):1255-8. doi: 10.1016/0020-7519(95)00050-c.
Dictyocaulus viviparus-infected calves exhibit strong antibody responses to the surface of all stages of the parasite. To examine whether surface-bound antibody is released, fluorescent-labelled antibody was bound to living parasites that were incubated either at 37 degrees C, or in the presence of a metabolic inhibitor at 2 degrees C. The amount of surface-bound antibody was measured by quantitative fluorescence before and after incubation for 24 h. Loss of antibody from the parasite surface was compared in adult worms, sheathed and artificially exsheathed third-stage larvae (L3). Rapid reductions in fluorescence were observed with adult parasites maintained at 37 degrees C but this was inhibited by incubation at 2 degrees C in the presence of sodium azide. In contrast, there was no such loss from the surface of the sheathed or exsheathed L3 maintained at 37 degrees C.
感染胎生网尾线虫的犊牛对该寄生虫各发育阶段的表面呈现出强烈的抗体反应。为检测表面结合抗体是否会释放，将荧光标记抗体与活寄生虫结合，分别在37℃孵育，或在2℃存在代谢抑制剂的条件下孵育。孵育24小时前后，通过定量荧光测定表面结合抗体的量。比较了成虫、带鞘和人工脱鞘的第三期幼虫（L3）表面抗体的损失情况。维持在37℃的成虫寄生虫表面荧光迅速降低，但在2℃存在叠氮化钠的情况下孵育可抑制这种降低。相比之下，维持在37℃的带鞘或脱鞘L3表面没有这种抗体损失。
