利用傅里叶变换拉曼光谱对人和羊骨进行结构评估并与合成羟基磷灰石作比较

Structural evaluation of human and sheep bone and comparison with synthetic hydroxyapatite by FT-Raman spectroscopy.

作者信息

Rehman I, Smith R, Hench L L, Bonfield W

机构信息

Interdisciplinary Research Centre in Biomedical Materials, Queen Mary and Westfield College, University of London, United Kingdom.

出版信息

J Biomed Mater Res. 1995 Oct;29(10):1287-94. doi: 10.1002/jbm.820291016.

DOI:10.1002/jbm.820291016

PMID:8557731

Abstract

The composition of whole human and sheep cortical bone tissue, and of a synthetic hydroxyapatite (P120), were compared using Fourier transform Raman (FT-Raman) spectroscopy. Deproteination procedures to remove the bulk of the collagen present in bone tissue allowed isolation of the mineral phase. A comparison of the spectra obtained from both whole and deproteinated bone with those of synthetic hydroxyapatite showed direct correlation only in the region of 952 cm-1 (symmetric P-O mode). In contrast, human and sheep bone were very closely matched in both, the organic and inorganic structures. The results demonstrate that deproteination of bone is not a necessary precursor to obtain spectral information.

摘要

利用傅里叶变换拉曼（FT - 拉曼）光谱对完整的人类和绵羊皮质骨组织以及一种合成羟基磷灰石（P120）的成分进行了比较。通过脱蛋白程序去除骨组织中大部分存在的胶原蛋白，从而分离出矿物相。将完整骨和脱蛋白骨所获得的光谱与合成羟基磷灰石的光谱进行比较，结果显示仅在952 cm-1区域（对称P - O模式）存在直接相关性。相比之下，人类和绵羊骨在有机和无机结构方面都非常匹配。结果表明，对骨进行脱蛋白并非获取光谱信息的必要前提。