Castro A J, Clegg D A, McCLUNG J R
Am J Anat. 1977 May;149(1):39-46. doi: 10.1002/aja.1001490104.
Many previous reports have demonstrated the development of aberrant neural connections in response to neonatal brain lesions. This investigation was undertaken to study possible alterations, particularly axonal sprouting, in rodent rubrospinal projections after neonatal destruction of the corticospinal tract through frontal cortical ablation. The neonatal ablations were made by aspiration in 1 to 2-day-old rats under hypothermic anesthesia. At three to six months after neonatal surgery, the rubrospinal tracts were ablated bilaterally in these small animals as well as in controls, by stereotaxically transecting the ventral tegmental decussation. Animals were killed two to six days after adult surgery, and rubrospinal projections were demonstrated using the Fink-Heimer degeneration stain. No differences in the pattern of rubrospinal projections were observed between animals with neonatal cortical lesions and controls. In all animals rubrospinal projections were located primarily in Rexed's lamina VI with a slight distribution into lamina V and the dorsal portion of lamina VII. Various hypotheses explaining the lack of rubrospinal sprouting after neonatal cortical lesions are presented, along with possible experiments to test these hypotheses.
许多先前的报告已经证明，新生儿脑损伤会导致异常神经连接的形成。本研究旨在探讨在新生大鼠通过额叶皮质切除破坏皮质脊髓束后，红核脊髓投射可能发生的改变，特别是轴突发芽。新生大鼠的切除手术在低温麻醉下，对1至2日龄的大鼠进行抽吸。在新生手术后三至六个月，通过立体定向横断腹侧被盖交叉，对这些小动物以及对照组动物的双侧红核脊髓束进行切除。成年手术后两至六天处死动物，使用芬克-海默变性染色法显示红核脊髓投射。在有新生儿皮质损伤的动物和对照组之间，未观察到红核脊髓投射模式的差异。在所有动物中，红核脊髓投射主要位于 Rexed 板层 VI，少量分布于板层 V 和板层 VII 的背侧部分。本文提出了各种解释新生儿皮质损伤后红核脊髓未发生发芽的假说，并给出了可能用于验证这些假说的实验。
