Gritti A, Parati E A, Cova L, Frolichsthal P, Galli R, Wanke E, Faravelli L, Morassutti D J, Roisen F, Nickel D D, Vescovi A L
Laboratory of Cellular Neuropharmacology, National Neurological Institute C, Milan, Italy.
J Neurosci. 1996 Feb 1;16(3):1091-100. doi: 10.1523/JNEUROSCI.16-03-01091.1996.
It has been established that the adult mouse forebrain contains multipotential (neuronal/glial) progenitor cells that can be induced to proliferate in vitro when epidermal growth factor is provided. These cells are found within the subventricular zone of the lateral ventricles, together with other progenitor cell populations, whose requirements for proliferation remain undefined. Using basic fibroblast growth factor (bFGF), we have isolated multipotential progenitors from adult mouse striatum. These progenitors proliferate and can differentiate into cells displaying the antigenic properties of astrocytes, oligodendrocytes, and neurons. The neuron-like cells possess neuronal features, exhibit neuronal electrophysiological properties, and are immunoreactive for GABA, substance P, choline acetyl-transferase, and glutamate. Clonal analysis confirmed the multipotency of these bFGF-dependent cells. Most significantly, subcloning experiments demonstrated that they were capable of self-renewal, which led to a progressive increase in population size over serial passaging. These results demonstrate that bFGF is mitogenic for multipotential cells from adult mammalian forebrain that possess stem cell properties.
业已确定，成年小鼠前脑含有多能（神经元/神经胶质）祖细胞，当提供表皮生长因子时，这些祖细胞可在体外被诱导增殖。这些细胞存在于侧脑室的室下区，与其他祖细胞群体在一起，其增殖需求尚不清楚。利用碱性成纤维细胞生长因子（bFGF），我们从成年小鼠纹状体中分离出了多能祖细胞。这些祖细胞能够增殖，并可分化为具有星形胶质细胞、少突胶质细胞和神经元抗原特性的细胞。类神经元细胞具有神经元特征，表现出神经元电生理特性，并且对γ-氨基丁酸（GABA）、P物质、胆碱乙酰转移酶和谷氨酸呈免疫反应性。克隆分析证实了这些依赖bFGF的细胞具有多能性。最重要的是，亚克隆实验表明它们能够自我更新，这导致在连续传代过程中细胞群体大小逐渐增加。这些结果表明，bFGF对具有干细胞特性的成年哺乳动物前脑多能细胞具有促有丝分裂作用。
