Dorsal spinocerebellar tract neurons in the chronic intact cat during wakefulness and sleep: analysis of spontaneous spike activity.

Relatively little is known about the transmission of ascending sensory information from lumbar levels across the behavioral states of sleep and wakefulness. The present study used extracellular recording methods in chronically instrumented intact behaving cats to monitor the activity of lumbar dorsal spinocerebellar tract (DSCT) neurons within Clarke's column during the states of wakefulness, quiet sleep, and active sleep. Clarke's column DSCT neurons were identified using antidromic identification and retrograde labeling techniques. The spontaneous spike rate and interspike interval data of DSCT neurons were quantified as a function of behavioral state. During wakefulness and quiet sleep, the spike rate of DSCT neurons was stable, and interspike interval histograms (ISIH) indicated a relatively high degree of regularity in DSCT neuronal spike train patterns. In contrast, during active sleep there was a marked reduction in the ongoing spike rate in a vast majority of cells tested. The magnitude of change in ISIHs and interspike interval data during active sleep depended in part on whether the reduction in cell firing was maintained or periodic throughout active sleep. Further suppression of spontaneous activity also was observed during intense rapid-eye-movement episodes of active sleep that were associated with clustered pontogeniculo-occipital wave and muscular twitches and jerks. After re-awakening, spontaneous spike activity of Clarke's column DSCT neurons resembled that recorded during previous episodes of wakefulness. These data provide evidence that ascending proprioceptive and exteroceptive sensory transmission through Clarke's column is diminished during the behavioral state of active sleep.