Soja P J, Fragoso M C, Cairns B E, Jia W G
Division of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada.
J Neurosci. 1996 Feb 1;16(3):1260-72. doi: 10.1523/JNEUROSCI.16-03-01260.1996.
Relatively little is known about the transmission of ascending sensory information from lumbar levels across the behavioral states of sleep and wakefulness. The present study used extracellular recording methods in chronically instrumented intact behaving cats to monitor the activity of lumbar dorsal spinocerebellar tract (DSCT) neurons within Clarke's column during the states of wakefulness, quiet sleep, and active sleep. Clarke's column DSCT neurons were identified using antidromic identification and retrograde labeling techniques. The spontaneous spike rate and interspike interval data of DSCT neurons were quantified as a function of behavioral state. During wakefulness and quiet sleep, the spike rate of DSCT neurons was stable, and interspike interval histograms (ISIH) indicated a relatively high degree of regularity in DSCT neuronal spike train patterns. In contrast, during active sleep there was a marked reduction in the ongoing spike rate in a vast majority of cells tested. The magnitude of change in ISIHs and interspike interval data during active sleep depended in part on whether the reduction in cell firing was maintained or periodic throughout active sleep. Further suppression of spontaneous activity also was observed during intense rapid-eye-movement episodes of active sleep that were associated with clustered pontogeniculo-occipital wave and muscular twitches and jerks. After re-awakening, spontaneous spike activity of Clarke's column DSCT neurons resembled that recorded during previous episodes of wakefulness. These data provide evidence that ascending proprioceptive and exteroceptive sensory transmission through Clarke's column is diminished during the behavioral state of active sleep.
关于从腰椎水平向上传递的感觉信息在睡眠和清醒行为状态之间的传输，人们了解得相对较少。本研究采用细胞外记录方法，对长期植入仪器的完整行为猫进行监测，以观察在清醒、安静睡眠和活跃睡眠状态下，克拉克柱内腰髓背侧脊髓小脑束（DSCT）神经元的活动。使用逆向鉴定和逆行标记技术来识别克拉克柱DSCT神经元。将DSCT神经元的自发放电率和峰间期数据作为行为状态的函数进行量化。在清醒和安静睡眠期间，DSCT神经元的放电率稳定，峰间期直方图（ISIH）表明DSCT神经元放电序列模式具有较高的规律性。相比之下，在活跃睡眠期间，绝大多数测试细胞的持续放电率显著降低。活跃睡眠期间ISIHs和峰间期数据的变化幅度部分取决于细胞放电的减少在整个活跃睡眠期间是持续的还是周期性的。在与成群的脑桥膝状体枕叶波以及肌肉抽搐和痉挛相关的活跃睡眠快速眼动强烈发作期间，还观察到自发放电活动的进一步抑制。重新唤醒后，克拉克柱DSCT神经元的自发放电活动类似于之前清醒期间记录的情况。这些数据提供了证据，表明在活跃睡眠行为状态期间，通过克拉克柱的本体感觉和外感受感觉的上行传输会减少。
