Beard J L, Burton J W, Theil E C
Department of Human Nutrition, Pennsylvania State University, University Park 16802-0001, USA.
J Nutr. 1996 Jan;126(1):154-60. doi: 10.1093/jn/126.1.154.
Ferritin and soybean meal were reevaluated as dietary treatments of iron deficiency in rats. Isotopes that had been used in the past were avoided because of contemporary knowledge of the physiological and structural complexity of ferritin protein and the solid iron mineral. Rats made anemic by iron-deficient diets were given equivalent amounts of iron as FeSO4, horse spleen ferritin, baked soybean meal, or soybean meal plus ferritin. Full recovery (89-109%) from anemia and increased tissue iron occurred after 28 d of treatment with any of the iron sources, which contrasts to past bioavailability studies using 59Fe-labeled ferritin and generally shorter periods of observation. Cultivar-specific variability was observed in soybean seed soluble iron and ferritin content (1.9-2.0 times the control cultivar, Arksoy), which was apparently heritable. The combined data suggest that manipulating ferritin expression and other soluble components of seed iron in soybeans and possibly other seeds, using Mendelian and biotechnological approaches, could contribute to a sustainable solution to global problems of iron deficiency.
对铁蛋白和豆粕作为大鼠缺铁饮食疗法进行了重新评估。由于当前对铁蛋白蛋白质和固态铁矿物质的生理及结构复杂性的了解，避免使用过去曾用过的同位素。用缺铁饮食导致贫血的大鼠分别给予等量的铁，形式为硫酸亚铁、马脾铁蛋白、烘焙豆粕或豆粕加铁蛋白。用任何一种铁源治疗28天后，贫血完全恢复（89 - 109%）且组织铁增加，这与过去使用59Fe标记铁蛋白且观察期通常较短的生物利用度研究形成对比。在大豆种子可溶性铁和铁蛋白含量方面观察到品种特异性差异（是对照品种Arksoy的1.9 - 2.0倍），这显然具有遗传性。综合数据表明，利用孟德尔和生物技术方法调控大豆以及可能其他种子中铁蛋白的表达和种子铁的其他可溶性成分，可能有助于可持续解决全球缺铁问题。
