Aoyama T, Fukui K, Taniguchi K, Nagaoka S, Yamaoto T, Hashimoto Y
Department of Nutrition and Health, Fuji Oil Co., Ltd., Osaka, Japan.
J Nutr. 1996 Jan;126(1):225-31. doi: 10.1093/jn/126.1.225.
This study determined the effect of dietary (dipalmitoyl) triacylglycerol (TAG) structure on the absorption and metabolism of fat in the presence or absence of cholesterol. Sprague-Dawley male rats weighing approximately 180 g were fed diets containing 10 g/100 g diet 1,3-dipalmitoyl-2-oleoyl-glycerol-rich oil (POP) or 1(3),2-dipalmitoyl-3(1)-oleoyl-glycerol-rich oil (PPO), either with or without cholesterol supplementation (0.5 g/100 g diet), for 2 wk. There were no differences in the growth of rats fed the various diets. The apparent absorption efficiency of dietary fat especially the apparent absorption efficiency of palmitic acid, was significantly lower in the POP groups than in the groups fed the PPO diets. The excretion of cholesterol in feces of rats fed the POP diet with cholesterol was significantly greater than those fed the PPO diet containing cholesterol. The excretion of total bile acids in the rats fed cholesterol was significantly greater than in those fed the diets without cholesterol. Liver cholesterol was significantly greater in rats fed cholesterol and was not affected by the TAG structure. Plasma cholesterol was significantly lower in the POP dietary groups than in the PPO groups. These results suggest that the absorption and metabolism of fat depend on the positional isomer of the fatty acid.
本研究确定了在有或没有胆固醇存在的情况下，膳食（二棕榈酰）三酰甘油（TAG）结构对脂肪吸收和代谢的影响。给体重约180 g的Sprague-Dawley雄性大鼠喂食含有10 g/100 g膳食的富含1,3-二棕榈酰-2-油酰甘油的油（POP）或富含1(3),2-二棕榈酰-3(1)-油酰甘油的油（PPO）的日粮，添加或不添加胆固醇（0.5 g/100 g膳食），持续2周。喂食不同日粮的大鼠生长情况没有差异。POP组膳食脂肪的表观吸收效率，尤其是棕榈酸的表观吸收效率，显著低于喂食PPO日粮的组。喂食含胆固醇的POP日粮的大鼠粪便中胆固醇的排泄量显著高于喂食含胆固醇的PPO日粮的大鼠。喂食胆固醇的大鼠总胆汁酸的排泄量显著高于喂食不含胆固醇日粮的大鼠。喂食胆固醇的大鼠肝脏胆固醇显著更高，且不受TAG结构的影响。POP日粮组的血浆胆固醇显著低于PPO组。这些结果表明，脂肪的吸收和代谢取决于脂肪酸的位置异构体。
