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利用聚合酶链反应技术对低密度脂蛋白受体基因敲除小鼠进行快速基因分型。

Rapid genotyping of low density lipoprotein receptor knockout mice using a polymerase chain reaction technique.

作者信息

Gaw A, Mancini F P, Ishibashi S

机构信息

Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas 75235, USA.

出版信息

Lab Anim. 1995 Oct;29(4):447-9. doi: 10.1258/002367795780739944.

DOI:10.1258/002367795780739944
PMID:8558830
Abstract

A new method for the rapid genotyping of low density lipoprotein receptor knockout mice is described. This method using a polymerase chain reaction (PCR) technique may be performed on small tissue biopsies, and represents a significant advantage in time over conventional genotyping by Southern blot analyses.

摘要

本文描述了一种用于低密度脂蛋白受体基因敲除小鼠快速基因分型的新方法。这种使用聚合酶链反应（PCR）技术的方法可用于小组织活检样本，与传统的Southern印迹分析基因分型相比，在时间上具有显著优势。

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