Horváth A, Sántha P, Horváth V, Török Nóra, Nagy I, Jancsó G, Vágvölgyi Cs, Somogyvári F
Department of Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.
Acta Biol Hung. 2013 Jun;64(2):262-5. doi: 10.1556/ABiol.64.2013.2.11.
A new, rapid method is described which permits the genotyping of genetically modified animals from a microlitre volume of whole blood samples via one step polymerase chain reaction amplification. The major advantage of the presented method is the exclusion of a DNA preparation step, which significantly reduces the time expenditure and work load of the genetic testing. Pilot studies indicate, that this method is efficient and applicable also on tissue biopsies and larger amount of blood providing a rapid and reliable new technique over conventional genotyping approaches.
本文描述了一种新的快速方法，该方法可通过一步聚合酶链反应扩增，从微升全血样本中对转基因动物进行基因分型。该方法的主要优点是无需DNA制备步骤，这显著减少了基因检测的时间消耗和工作量。初步研究表明，该方法高效且也适用于组织活检和大量血液样本，为传统基因分型方法提供了一种快速可靠的新技术。
