Mohammad R M, Mohamed A N, Hamdan M Y, Vo T, Chen B, Katato K, Abubakr Y A, Dugan M C, al-Katib A
Department of Internal Medicine, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Leukemia. 1996 Jan;10(1):130-7.
Chronic lymphocytic leukemia (CLL), a proliferative disease of mature looking B lymphocytes, is the commonest leukemia in western countries. It remains incurable by available treatment modalities. We report on the establishment of a permanent, EBV-negative, B-CLL line (WSU-CLL) from the peripheral blood of a patient with CLL. The cells grow as suspension in liquid culture, express IgG lambda and other B cell markers and show lg heavy and light gene rearrangements. Karyotypic analysis shows 45,X,del(3)(p14;p24),t(4;12;12) (q31;q22;p13), t(5;12) (q31;p13), add(16)(q24)X2, t(18;21) (q12;p12). WSU-CLL forms colonies when grown on soft agar. A xenograft model was established by injecting the WSU-CLL cells subcutaneously (s.c.) in severe combined immune deficient (SCID) mice. When the s.c. tumor was transplanted in vivo to other SCID mice, the success rate was 100% with a doubling time of 7.3 days. The CLL-SCID xenograft model was used to test the efficacy of selected standard chemotherapy drugs and new therapeutic agents against WSU-CLL. The cell line and the xenograft described can be used as a model to facilitate the development of new therapeutic agents against CLL in man.
慢性淋巴细胞白血病（CLL）是一种成熟外观的B淋巴细胞增殖性疾病，是西方国家最常见的白血病。现有治疗方式仍无法治愈该病。我们报告了从一名CLL患者外周血中建立了一个永久性、EBV阴性的B-CLL细胞系（WSU-CLL）。这些细胞在液体培养中呈悬浮生长，表达IgG λ和其他B细胞标志物，并显示Ig重链和轻链基因重排。核型分析显示为45,X,del(3)(p14;p24),t(4;12;12) (q31;q22;p13), t(5;12) (q31;p13), add(16)(q24)X2, t(18;21) (q12;p12)。WSU-CLL在软琼脂上生长时可形成集落。通过将WSU-CLL细胞皮下注射到严重联合免疫缺陷（SCID）小鼠中建立了异种移植模型。当将皮下肿瘤体内移植到其他SCID小鼠时，成功率为100%，倍增时间为7.3天。CLL-SCID异种移植模型用于测试选定的标准化疗药物和新治疗剂对WSU-CLL的疗效。所描述的细胞系和异种移植可作为一种模型，以促进针对人类CLL的新治疗剂的开发。
