Dirks W G, Zaborski M, Jäger K, Challier C, Shiota M, Quentmeier H, Drexler H G
Department of Human and Animal Cell Cultures, Braunschweig, Germany.
Leukemia. 1996 Jan;10(1):142-9.
The CD30+ anaplastic large cell lymphoma (ALCL) represents a new lymphoma entity thought to be related to Hodgkin'S disease (HD), but displaying also its own unique features. Cytogenetic studies of ALCL have demonstrated the presence of a (2;5)(p23;q35) translocation in a substantial number of these cases. Recently, the t(2;5) has been cloned and described to represent fusion of the NPM gene with the ALK gene on chromosome 5. To better define the spectrum of lymphomas containing this abnormality we have analyzed 50 continuous human cell lines established from various types of non-Hodgkin's lymphoma, ALCL and HD. In a first step, the expression of the NPM-ALK fusion gene was examined by reverse transcriptase-polymerase chain reaction (RT-PCR). In a second step, the t(2;5)-carrying cells were tested for the translation of functional chimeric mRNA into a fusion protein by immuno-staining of single cells with a polyclonal antibody. The NPM-ALK fusion transcript and the p80 protein were detected in eight of nine ALCL cell lines. We were unable to find PCR evidence for the t(2;5) in any of the non-ALCL cell lines including other CD30+ cell lines. As all seven bona fide HD cell lines were NPM-ALK-negative, these results do not support the notion that the t(2;5) represents a chromosomal aberration common to both ALCL and HD.
CD30+间变性大细胞淋巴瘤（ALCL）是一种新的淋巴瘤实体，被认为与霍奇金病（HD）有关，但也有其自身独特的特征。对ALCL的细胞遗传学研究表明，在相当数量的此类病例中存在(2;5)(p23;q35)易位。最近，t(2;5)已被克隆并描述为代表5号染色体上NPM基因与ALK基因的融合。为了更好地界定含有这种异常的淋巴瘤谱，我们分析了从各种类型的非霍奇金淋巴瘤、ALCL和HD建立的50个人类连续细胞系。第一步，通过逆转录聚合酶链反应（RT-PCR）检测NPM-ALK融合基因的表达。第二步，通过用多克隆抗体对单细胞进行免疫染色，检测携带t(2;5)的细胞中功能性嵌合mRNA是否翻译成融合蛋白。在9个ALCL细胞系中的8个中检测到了NPM-ALK融合转录本和p80蛋白。在包括其他CD30+细胞系在内的任何非ALCL细胞系中，我们都未能找到t(2;5)的PCR证据。由于所有7个真正的HD细胞系均为NPM-ALK阴性，这些结果不支持t(2;5)代表ALCL和HD共有的染色体畸变这一观点。
